Impact of the biocontrol agent Trichoderma atroviride SC1 on soil microbial communities of a vineyard in northern Italy |
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| Authors: | Federica Savazzini Claudia Maria Oliveira Longa Ilaria Pertot |
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| Institution: | Fondazione Edmund Mach, Plant Protection Department, 38010 S. Michele all'Adige TN, Italy |
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| Abstract: | The fungus Trichoderma atroviride SC1 is an experimental biocontrol agent (BCA) that is active against the fungus Armillaria mellea. Following the application of Trichoderma to the surface soil of a vineyard, we used a highly specific real-time PCR, previously validated for the analysis of soil microcosms, to monitor the populations of this fungus at different soil depths over several months. The quantification obtained using this molecular method was highly correlated with laboratory assays of colony-forming units. The native communities of bacteria and fungi in the soil were analyzed using automated ribosomal intergenic spacer analysis (ARISA), and transient changes were observed following the application of T. atroviride SC1 conidia. A principal component of variance analysis demonstrated that the introduction of T. atroviride SC1 had an effect on the soil microflora during the first two weeks following inoculation. However, at later dates, environmental conditions had a higher influence on the surveyed communities than the BCA application, as confirmed through the use of the Shannon index of biodiversity. Soil depth had a strong influence on the composition and biodiversity of fungal communities. |
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| Keywords: | Trichoderma Biocontrol Risk assessment Microbial community structure ARISA Real-time PCR |
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