| 基于ISSR分析28份香蕉种质的基因组DNA多样性 |
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| 引用本文： | 郭计华,李新国,李丽,李绍鹏,罗轩,魏守兴,谢子四.基于ISSR分析28份香蕉种质的基因组DNA多样性[J].广西农业生物科学,2012(5):492-497. |
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| 作者姓名： | 郭计华 李新国 李丽 李绍鹏 罗轩 魏守兴 谢子四 |
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| 作者单位： | [1]热带作物种质资源保护与开发利用教育部重点实验室,海南大学园艺园林学院,海口570228 [2]中国热带农业科学院热带作物品种资源所,儋州571737 |
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| 基金项目： | 国家自然科学基金（30860170,31260462）; 海南大学植物学国家重点学科（071001）; 海南省自然科学基金（807034）; 热带作物种质资源保护与开发利用教育部重点实验室（2011hckled-13）共同资助 |
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| 摘 要： | 利用ISSR标记技术分析了28份香蕉种质的遗传多态性。从100个ISSR引物中筛选出8个多态性引物,共扩增出55条DNA带,其中46条为多态性带,占83.6%,平均每个引物扩增的DNA带数为6.88条。依相似系数0.73的水平,将香蕉28个品种划分为6大类。其中云南BB（BB）和东莞高把大蕉（ABB）在相似系数为0.94时,二者的亲缘关系较近。Pisang Ceylan（AAB）和FHIA-18（AAAB）相似系数水平接近为1,表明二者亲缘关系最近。本研究为香蕉遗传关系的建立及品种鉴定提供理论基础。
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| 关 键 词： | 香蕉 ISSR 多样性 |
Based on the ISSR Analysis of Genomic DNA Diversity of 28 Banana Germplasms |
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| Guo Jihua,Li Xinguo,Li Li,Li Shaopeng,Luo Xuan,Wei Shouxing,Xie Zisi.Based on the ISSR Analysis of Genomic DNA Diversity of 28 Banana Germplasms[J].Journal of Guangxi Agricultural and Biological Science,2012(5):492-497. |
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| Authors: | Guo Jihua Li Xinguo Li Li Li Shaopeng Luo Xuan Wei Shouxing Xie Zisi |
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| Institution: | 1 Key Laboratory of Protection and Developmental Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Horticulture and Landscape Architecture, Hainan University, Haikou, 570228; 2 Tropical Crops Genetic Resource Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, 571737 |
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| Abstract: | Using ISSR markers analysis of 28 banana germplasms genetic polymorphism. Eight primers were screened from 100 arbitrary primers, and a total of 55 DNA bands were amplified, among which 46 （83.6%） were polymorphic. The average number of DNA bands amplified by each primer was 6.88. The similarity coefficient of 0.73 near which divided the 28 varieties into six groups. Including yunnan BB （BB） and the dongguan high the plantain （ABB） in the similarity coefficient is 0.94, the two have close kin. Pisang Ceylan （AAB） and FHIA-18 （AAAB） are closest relative, similarity coefficient level close to 1. This study provided the theoretical basis for banana genetic relationship building and variety identification . |
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| Keywords: | Banana ISSR Diversity |
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