| 利用MSAP分析18个芥蓝齐口期的表观遗传多样性 |
| |
| 引用本文： | 史卫东,黄如葵,陈振东,罗海玲,康红卫,梁家作,刘杏连.利用MSAP分析18个芥蓝齐口期的表观遗传多样性[J].广西农业生物科学,2012(5):505-512. |
| |
| 作者姓名： | 史卫东 黄如葵 陈振东 罗海玲 康红卫 梁家作 刘杏连 |
| |
| 作者单位： | 广西农科院蔬菜研究所,南宁530007 |
| |
| 基金项目： | 广西农业科学院基本科研业务专项（2012JZ06和桂农科2012YT05）; 广西作物遗传改良生物技术重点实验室开放课题子课题（桂科能0815011-6-1）共同资助 |
| |
| 摘 要： | 本研究利用MSAP检测18个芥蓝齐口期DNA甲基化水平,分析了表观遗传多样性,探讨DNA甲基化模式对齐口期的影响。结果表明,18个芥蓝齐口期平均为50d,叶片数平均为10片,齐口期和叶片数不相关（相关系数为0.296）;变异系数分别为21%和18%;遗传距离分布在0~40,平均值为12.2276,在10.62处分为3类。MSAP分析表明,5对引物组合扩增得到432条多态性条带,201条片段表现出多态性,多态性比率为47%;Nei遗传距离分布在0.004~0.467,平均值为0.0958,表明遗传多样性水平较低;在0.04处分为3类。Mantel测验表明两种分析的遗传距离相关系数为-0.1366,显示齐口期、叶片数与DNA甲基化多态性没有相关性。DNA甲基化模式分析表明,非甲基化片段为110条,甲基化多态性片段为322条,分为3种带型,类型一为非甲基化带型（110条）,类型二为甲基化带型（110条）,类型三为半甲基化带型（152条）,非甲基化片段和半甲基化片段在不同品种之间呈现多态性,甲基化片段在不同品种之间呈现多态性与单态性相差不大,显示MSAP多态性主要来源于非甲基化和半甲基化片段,芥蓝甲基化模式以半甲基化为主。本文推测DNA甲基化水平降低参与芥蓝齐口期调控,MSAP分析既可用于基因组结构研究,又可用于基因组水平上性状的功能研究。
|
| 关 键 词： | 芥蓝 表观遗传多样性 MSAP分析 |
The Epigenetic Genetic Diversity of 18 Chinese Kales Analyzed by MSAP when Initiated Flowering |
| |
| Shi Weidong Huang Ruikui Chen Zhendong Luo Hailing Kang Hongwei Liang Jiazuo Liu Xinglian.The Epigenetic Genetic Diversity of 18 Chinese Kales Analyzed by MSAP when Initiated Flowering[J].Journal of Guangxi Agricultural and Biological Science,2012(5):505-512. |
| |
| Authors: | Shi Weidong Huang Ruikui Chen Zhendong Luo Hailing Kang Hongwei Liang Jiazuo Liu Xinglian |
| |
| Institution: | Shi Weidong Huang Ruikui Chen Zhendong Luo Hailing Kang Hongwei Liang Jiazuo Liu Xinglian The Institute of Vegetable of Guangxi Academy of Agricultural Sciences, Nanning, 530007 |
| |
| Abstract: | The DNA methylation level of 18 Chinese kale in the initial flowering stage was detected by MSAP in order to analyze the epigenetic genetic diversity and impact of DNA methylation. The phenotype results showed that average initial flowering time and leaf numbers was 50 days and 10 individually and there was no relationship （Correlation coefficient was 0.296.）. Coefficient of variability was 21% and 18% individually. Genetic distance varied between 0~40 and average was 12.227 6. Three classes were grouped at genetic distance 10.62. 432 bands were amplified with 5 EcoRⅠ＋Hpa Ⅱ and EcoRⅠ＋MspⅠprimer combinations in MSAP. 201 bands were polymorphic bands and polymorphism percentage was 47%. Nei＇s genetic distance varied 0.004~0.467 and average was 0.095 8, so one can conclude that genetic diversity was relatively low in Chinese kale. Three classes were grouped at genetic distance 0.04. Correlation coefficient was -0.136 6 between phenotype and MSAP genetic distance, so there wasn＇t correlation between initial flowering time combined with leaf numbers and DNA methylation polymorphism. In DNA pattern analysis monomorphic methylation bands were 110 and polymorphic methylation bands were 322 of which three clusters were grouped. The first 110 was no methylation （EcoRⅠ＋MspⅠ/ EcoRⅠ＋Hpa Ⅱ）. The second 110 was methylation bands digested with EcoRⅠ＋MspⅠ. The third 152 was hypomethylation band digested with EcoRⅠ＋Hpa Ⅱ. The nomethylation and hypomethylation bands displayed more between varieties compared with less methylation bands mean the methylation polymorphism came from nomethylation and hypomethylation bands, hypomethylation was major methylation type in kales. As a result one can speculation DNA methylation decreasd was related to initial flowering. The research also proved MSAP analysis can also be used functional genomics research except for structural genomics. |
| |
| Keywords: | Chinese kale（Brassica oleracea L var alboglabra Bailey） Epigenetic genetic diversity Methylation-sensitive AFLP markers（MSAP） |
| 本文献已被 维普 等数据库收录！ |
|
