| 牛冻、鲜精子差异蛋白的双向电泳和质谱鉴定的初步研究 |
| |
| 引用本文： | 吴承疆,朱化彬,王栋,郝海生,杜卫华,赵学明,路永强.牛冻、鲜精子差异蛋白的双向电泳和质谱鉴定的初步研究[J].中国畜牧兽医,2010,37(11):100-104. |
| |
| 作者姓名： | 吴承疆 朱化彬 王栋 郝海生 杜卫华 赵学明 路永强 |
| |
| 作者单位： | （1.吉林农业大学动物科学技术学院，长春 130118；2.中国农业科学院北京畜牧兽医研究所，北京 100193；3.北京市畜牧兽医总站，北京 100107） |
| |
| 基金项目： | 国家自然科学基金资助项目，国家"863"高技术研究发展规划 |
| |
| 摘 要： | 采用适合于精子裂解的热Trizol法制备牛精子全蛋白质，应用线性固相IPG胶条（pH 3～7、24 cm）进行精子全蛋白2-DE电泳，在冻精和鲜精蛋白图谱中分别检测到839±34个蛋白点和564±16个蛋白点，经差异比较和显著性检验，在冻、鲜精子中共得到19个具有显著差异的蛋白点，在冻精中表达上调的蛋白点有9个，表达下调的蛋白点有1个，只在冻精中表达的蛋白点有9个。经过酶解和质谱鉴定，获得了3个差异蛋白点的质谱信息，生物信息学分析结果发现，这3个差异点分别为中性鞘磷脂酶激活结合因子（FAN）、谷胱甘肽S转移酶（GST-Mu5）及锌离子结合细胞色素氧化酶，分别与细胞应激和凋亡有关。推测认为，这3种与细胞应激反应及凋亡相关的蛋白在冻精中显著增高可能与精子冷冻损伤有关。
|
| 关 键 词： | 冻 鲜精子 2-DE 质谱分析 冷冻损伤 |
Primary Study on Identify of the Differential Proteins between Bovine Frozen and Fresh Spermatozoa Using 2-DE and MS |
| |
| WU Cheng-jiang,ZHU Hua-bin,WANG Dong,HAO Hai-sheng,DU Wei-hua,ZHAO Xue-ming,LU Yong-qiang.Primary Study on Identify of the Differential Proteins between Bovine Frozen and Fresh Spermatozoa Using 2-DE and MS[J].China Animal Husbandry & Veterinary Medicine,2010,37(11):100-104. |
| |
| Authors: | WU Cheng-jiang ZHU Hua-bin WANG Dong HAO Hai-sheng DU Wei-hua ZHAO Xue-ming LU Yong-qiang |
| |
| Institution: | (1.College Animal Science and Technology， Jilin Agricultural University， Changchun 130118， China； 2.Institute of Animal Sciences，Chinese Academy of Agricultural Sciences， Beijing 100193， China； 3.Beijing General Station of Animal Husbandry and Veterinary Medicine， Beijing 100107， China) |
| |
| Abstract: | Heat Trizol method was used to prepare the global protein of bovine spermatozoa, and the global proteins of fresh sperm and frozen sperm was separated by the technique of 2-DE with linear IPG (pH 3 to pH 7, 24 cm). 839±34 protein spots were obtained from the sample of frozen spermatozoa, while 564±16 from the sample of fresh spermatozoa. 19 differential proteins between frozen and fresh sperm were detected after analysis and significance test with software of ImageMasterTM 2D Platinum, 9 of which were expressed up-regulated while one of which was down-regulated in the sample of frozen spermatozoa. In addition, 9 spots were found only in frozen spermatozoa. After enzymolysis and MS detection, mass spectrum information of 3 differential proteins were obtained, and the differential proteins were considered to be neutral sphingomyelinase(N- SMase) activation associated factor(FAN), glutathione-S-transferase(Mu5) and zinc ion binding structure of bovine heart cytochrome c oxidase in the fully oxidized state, which were related to cell stress and apoptosis. It was presumed that the increasing of these proteins significantly in frozen spermatozoa were involved with cryodamage of spermatozoa. |
| |
| Keywords: | 2-DE |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
