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| 施磷对小麦旗叶氮代谢关键酶活性和子粒蛋白质含量的影响 | |
| 引用本文： | 王平,尹燕枰,付国占,郭营,蔡瑞国,梁太波,耿庆辉,邬云海,王振林.施磷对小麦旗叶氮代谢关键酶活性和子粒蛋白质含量的影响[J].植物营养与肥料学报,2009,15(1):24-31. |
| 作者姓名： | 王平 尹燕枰 付国占 郭营 蔡瑞国 梁太波 耿庆辉 邬云海 王振林 |
| 作者单位： | 1.山东农业大学农学院作物生物学国家重点实验室、农业部小麦栽培生理与遗传改良重点开放实验室,山东泰安 271018; |
| 基金项目： | 国家自然科学基金，教育部长江学者和创新团队发展计划，山东省自然科学基金 |
| 摘 要： | 在田间试验条件下，以强筋品种济麦20和弱筋品种山农1391为材料，研究了不同施磷水平对2类型品种小麦旗叶氮代谢关键酶活性和子粒蛋白质积累的效应。结果表明，在P2O5 0～135 kg /hm2范围内，施磷明显提高济麦20灌浆前期旗叶硝酸还原酶（NR）和谷氨酰胺合成酶（GS）活性；但却降低山农1391旗叶NR、GS活性。强筋品种济麦20在灌浆中、后期旗叶内肽酶（EP）、羧肽酶（CP）活性和蛋白质含量因施磷而提高；而弱筋品种山农1391的EP、CP活性和子粒蛋白质含量则降低。施磷使济麦20子粒清蛋白、醇溶蛋白和谷蛋白的含量提高，球蛋白含量有所降低；但弱筋品种山农1391子粒球蛋白、醇溶蛋白和谷蛋白的含量因施磷而降低，清蛋白含量未见显著变化。说明小麦施磷应考虑不同品质类型品种氮代谢及子粒蛋白质含量对施磷的反应。 |
| 关 键 词： | 小麦 磷素水平 酶活性 蛋白质含量 |
| 收稿时间： | 2007-12-5 |
Effect of phosphorus on activities of enzymes related to nitrogen metabolism in flag leaves and protein content of wheat grains |
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| WANG Ping,YIN Yan-ping,FU Gu-zhan,GUO Ying,CAI Rui-guo,LIANG Tai-bo,GENG Qing-hui,WU Yun-hai,WANG Zhen-lin.Effect of phosphorus on activities of enzymes related to nitrogen metabolism in flag leaves and protein content of wheat grains[J].Plant Nutrition and Fertilizer Science,2009,15(1):24-31. | |
| Authors: | WANG Ping YIN Yan-ping FU Gu-zhan GUO Ying CAI Rui-guo LIANG Tai-bo GENG Qing-hui WU Yun-hai WANG Zhen-lin |
| Institution: | 1.National Key Laboratory of Crop Biology,Agronomy College of Shandong Agricultural University,Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement,Ministry of Agriculture,Taian,Shandong,271018; 2 Agronomy College,Henan University of Science and Technology,Luoyang 471003,China; 3 Agronomy Department of Hebei Normal University of Science and Technology,Changli,Heibei 066600,China |
| Abstract: | Effect of phosphorus on the activities of enzymes related to nitrogen metabolism in flag leaves, and protein content in grains of wheat cultivars with different end-use quality were studied under field conditions. The studied enzymes include nitrate reductase (NR), glutamine synthetase (GS), endop eptidase (EP) and carboxyp eptidase (CP). Field experiment was conducted at the Experiment Station of Shandong Agricultural University in Taian, Shandong from 2005 through 2006. Two cultivars, strong gluten cultivar JM20 and weak gluten cultivar SN1391, were selected and three phosphorus application levels, P2O5 0, 135 and 225 kg/ha were set up. Results indicated that the effects of phosphorus application rates on enzyme activities and protein content varied with wheat cultivars. Both the enzyme activities and protein content in strong gluten wheat JM20 were promoted with increasing of phosphorus application rate, but declined in the weak gluten wheat SN1391. The un-unique response of different wheat cultivars on phosphorus should be fully considered during the process of developing P application strategy. |
| Keywords: | wheat phosphorus fertilizer rates enzyme activity protein content |
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