| 提取地衣真菌总DNA的简便方法 |
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| 引用本文： | 时向阳,魏江春.提取地衣真菌总DNA的简便方法[J].北京林业大学学报,1997,19(4):45-50. |
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| 作者姓名： | 时向阳 魏江春 |
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| 作者单位： | [1]北京林业大学森林资源与环境学院 [2]中国科学院微生物所真菌地衣系统学开放实验室 |
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| 摘 要： | 利用液氮破碎地衣体的上下表皮层及藻层，再用氯化苄在弱碱条件下破坏真菌细胞壁，从而获得地衣真菌总ＤＮＡ．此方法简便、省时、价廉，获得的总ＤＮＡ蛋白质污染少，产量高，可进一步进行ＰＣＲ扩增．
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| 关 键 词： | 地衣真菌，地衣体，总DNA，氯化苄，真菌细胞壁，PCR扩增 |
A Convenient Method of Extracting Total DNA from Lichen Fungus |
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| Shi Xiangyang,Wei Jiangchun,Jiang Yumei,Tian Suzhong,Guo Shouyu.A Convenient Method of Extracting Total DNA from Lichen Fungus[J].Journal of Beijing Forestry University,1997,19(4):45-50. |
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| Authors: | Shi Xiangyang Wei Jiangchun Jiang Yumei Tian Suzhong Guo Shouyu |
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| Institution: | Shi Xiangyang 1) Wei Jiangchun 2) Jiang Yumei 2) Tian Suzhong 3) Guo Shouyu 2) |
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| Abstract: | The process of the new method is that the upper or lower cortex and thaus of lichens are broken by Liquid Nitrogen,then fungi cell walls are destroyed by Benzyl Chloride(Phch 2Cl) under weak base condition,so the total DNA of lichen fungus is gained.The method is simple and convenient,so it can save both time and money.The gained total DNA has little miscellaneous proteins and DNA production is high.The DNA could be used for PCR amplification,RFLP analysis and sequencing analysis. |
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| Keywords: | lichen fungus lichens total DNA benzyl chloride(Phch 2Cl) fungi cell wall PCR amplification |
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