| 意大利蜜蜂蜂毒透明质酸酶基因的克隆与序列分析 |
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| 引用本文： | 沈立荣,张传溪,程家安.意大利蜜蜂蜂毒透明质酸酶基因的克隆与序列分析[J].浙江大学学报(农业与生命科学版),2002,28(3):289-292. |
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| 作者姓名： | 沈立荣 张传溪 程家安 |
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| 作者单位： | 浙江大学,应用昆虫学研究所,浙江,杭州,310029 |
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| 摘 要： | 从意蜂毒腺抽提总RNA,用RT-PCR获得蜂毒透明质酸酶(AmHya)基因,其核苷酸序列全长为1 149 bp.将AmHya氨基酸序列与中蜂蜂毒透明质酸酶(AcHya)和其它5种透明质酸酶(Hya)氨基酸序列比较,结果显示,AmHya与 AcHya的同源性最高(91%),同时对7种Hya作了分子结构与分子进化分析.
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| 关 键 词： | 意蜂 蜂毒 透明质酶 基因 序列分析 |
| 文章编号： | 1008-9209(2002)03-0289-04 |
| 修稿时间： | 2001年7月23日 |
Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mEllifera |
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| SHEN Li rong,ZHANG Chuan xi,CHENG Jia an.Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mEllifera[J].Journal of Zhejiang University(Agriculture & Life Sciences),2002,28(3):289-292. |
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| Authors: | SHEN Li rong ZHANG Chuan xi CHENG Jia an |
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| Abstract: | The venomous hyaluronidase (AmHya) gene of Italian honey bee ( Apis mellifera ) collected in China was amplified by RT PCR from total RNA of venom glands of the worker bee. Sequence analysis showed the gene was composed of 1 149 nucleotides . The alignment of the amino acid sequence of AmHya gene and 6 Hya genes from other organisms showed that AmHya is most closely related to the AcHya of Chinese honeybee ( A.cerana cerana ) with 91% amino acid level identity. A phylogenetic tree of 7 Hyaluronidases was drawn by using GENETYX program, and molecular structures and functions of these ensymes were compared and analysed. |
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| Keywords: | Apis mellifera bee venom hyaluronidase gene sequence analysis |
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