| 不同处理方式对奶牛粪细菌群落多样性及群落结构的影响 |
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| 引用本文： | 郑金伟,李辉信,胡锋.不同处理方式对奶牛粪细菌群落多样性及群落结构的影响[J].农业环境科学学报,2009,28(3). |
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| 作者姓名： | 郑金伟 李辉信 胡锋 |
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| 作者单位： | 南京农业大学资源与环境科学学院,江苏南京,210095 |
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| 基金项目： | 南京农业大学青年基金，国家高技术研究发展计划(863计划)，村镇垃圾处理与资源化技术项目 |
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| 摘 要： | 以腐熟牛粪、新鲜牛粪以及蚯蚓粪为材料,提取其微生物总DNA,利用细菌16S rDNA V3区扩增及变性梯度凝胶电泳(PCR-DGGE)法,分析了3种材料中细菌的多样性以及细菌群落结构的相似性.结果表明,蚯蚓粪的细菌多样性最丰富,腐熟牛粪次之,新鲜牛粪的细菌多样性最小.蚯蚓粪和腐熟牛粪的细菌群落结构有40%的相似性;蚯蚓粪和新鲜牛粪的细菌群落结构的相似性为25%;新鲜牛粪和腐熟牛粪的细菌群落结构的相似性则为35%.
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| 关 键 词： | 奶牛粪 蚯蚓堆制 微生物群落结构 |
Effects of Different Treat Ways on Microbial Diversity and Microbial Community Structure of Dairy Manure |
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| ZHENG Jin-wei,LI Hui-xin,HU Feng.Effects of Different Treat Ways on Microbial Diversity and Microbial Community Structure of Dairy Manure[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2009,28(3). |
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| Authors: | ZHENG Jin-wei LI Hui-xin HU Feng |
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| Institution: | College of Resources and Environmental Sciences;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | Effects of different treat ways on microbial diversity and microbial community structure of dairy manure were studied by PCRDGGE profiles of bacterial 16S rDNA V3 region.In this research, composted dairy manure and vermicompost came from fresh dairy manure by composting and vermicomposting.The total genomic DNA of composted dairy manure, fresh dairy manure and vermicompost were extracted respectively by direct DNA extraction method.By analyzing the DGGE profiles, it was found that the microbial diversity of... |
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| Keywords: | dairy manure vermicomposting microbial community structure |
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