| 橡胶树品种云研77-2茎芽诱导体系优化及移栽管理 |
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| 引用本文： | 孙小龙,和丽岗,梁国平,宁连云.橡胶树品种云研77-2茎芽诱导体系优化及移栽管理[J].热带作物学报,2009,30(11):1637-1640. |
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| 作者姓名： | 孙小龙 和丽岗 梁国平 宁连云 |
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| 作者单位： | 云南省热带作物科学研究所,云南景洪,666100 |
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| 摘 要： | 采用正交设计方法,研究橡胶树品种云研77-2茎段快繁试验中NAA、KT、6-BA 3种激素对茎芽诱导的影响.结果表明,培养基中添加6-BA对茎芽诱导起主导性的作用.橡胶树云研77-2茎芽诱导最适培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0～0.5 mg/L KT.诱导生根试验中,培养基为1/2 MS+0.5 mg/L IBA的生根率可达66.7%.叶片剪去1/2的自根无性系进行移栽的植株平均成活率为43.6%.
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| 关 键 词： | 橡胶树 组织培养 正交设计 |
Optimization of Inducing System for Stem Segments of Clone Yunyan77-2 of Rubber Tree (Hevea brasiliensis) and Its Transplanting Management |
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| Sun Xiaolong,He Ligang,Liang Guoping and Ning Lianyun.Optimization of Inducing System for Stem Segments of Clone Yunyan77-2 of Rubber Tree (Hevea brasiliensis) and Its Transplanting Management[J].Chinese Journal of Tropical Crops,2009,30(11):1637-1640. |
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| Authors: | Sun Xiaolong He Ligang Liang Guoping and Ning Lianyun |
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| Institution: | Yunnan Tropical Crops Research Institute;Yunnan Tropical Crops Research Institute;Yunnan Tropical Crops Research Institute;Yunnan Tropical Crops Research Institute |
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| Abstract: | Three hormones, NAA, KT and 6-BA, were used to induce stem segments for rapid propagation of clone Yunyan77-2, and an orthogonal design was arranged. The results showed that the medium in the presence of 8-BA had a dominant role in inducing shoots from the stem segments. The optimal shoot inducing medium was MS + 2.0 mg/L 6-BA + 0.1 mg/L NAA + (0～0.5) mg/L KT. The root inducing medium of 1/2MS + 0.5 mg/L IBA produced a rooting rate of 66.7%. The self-rooted plantlets with 1/2 leaves cut had a survival rate of 43.6% after transplanted. |
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| Keywords: | rubber tree tissue culture orthogonal design |
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