Low seminal plasma buffering capacity corresponds to high pH sensitivity of sperm motility in salmonids |
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| Authors: | RL Ingermann DC Bencic JG Gloud |
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| Institution: | (1) Department of Biological Sciences, Center for Reproductive Biology, University of Idaho, Moscow, ID 83844-3051, USA;(2) Present address: Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599-7090, USA |
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| Abstract: | The buffering capacity of air-equilibrated seminal plasma from the chinook salmon Oncorhynchus tshawytscha and steelhead Oncorhynchus mykiss were determined. Buffering capacities were significantly less at pH values at and above 7.3 than over the range 6.0 to 7.0
and were particularly low between pH values of approximately 7.5 to 8.5. The potential of chinook salmon sperm to become motile
upon addition to water was highly sensitive to pH. Only very low levels of motility were demonstrable when sperm had been
maintained at pH values at or below 7.5 prior to water exposure while maximum motility was noted when sperm had been maintained
at pH values of about 8.0 and above. Thus, the potential for motility appeared especially sensitive to that pH range over
which seminal plasma exhibited its lowest buffering capacity. Buffering capacity, protein content and inorganic phosphate
content of chinook salmon and steelhead seminal plasmas were less than those of corresponding blood plasma or ovarian fluid
suggesting that seminal plasma is unique among salmonid body fluids in its composition and low buffering capacity. This low
buffering capacity is likely to facilitate the role of the sperm duct epithelium in semen pH regulation and thus, in the sperm's
acquisition of the potential for motility upon exposure to water.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | buffer fish milt phosphate salmon semen |
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