| 瘦肉型与脂肪型猪血清Leptin的比较检测 |
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| 引用本文： | 龙火生,孙 超,庞卫军.瘦肉型与脂肪型猪血清Leptin的比较检测[J].西北农林科技大学学报(社会科学版),2004,32(6):5-8. |
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| 作者姓名： | 龙火生 孙 超 庞卫军 |
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| 作者单位： | 西北农林科技大学,动物科技学院,陕西,杨凌,712100 |
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| 基金项目： | 教育部"春晖计划"资助项目,西北农林科技大学重点科研项目 |
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| 摘 要： | 为了研究我国脂肪型猪与引入的瘦肉型猪ob基因的蛋白表达差异,用Western杂交比较检测了内江猪、八眉猪、长白猪和杜洛克猪的血清Leptin水平。结果发现,脂肪型猪体内存在Leptin抵抗现象,血清Leptin水平比瘦肉型猪高3.23倍;同时发现一个大小约为33ku的蛋白与Leptin有一定的结构同源性,该蛋白也可能是Leptin的二聚体;另外,脂肪型猪ob基因的表达高于瘦肉型猪,二者肥胖程度和瘦肉率性状的差异可能还存在与ob基因表达有关的机制。
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| 关 键 词： | 瘦肉型猪 脂肪型猪 Leptin SDS-PAGE Western杂交 |
| 文章编号： | 1671-9387(2004)06-0005-04 |
| 收稿时间： | 2003/5/29 0:00:00 |
| 修稿时间： | 2003年5月29日 |
Comparative detection of serum Leptin levels between obese and lean swine |
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| LONG Huo-sheng,SUN Chao,PANG Wei-jun,YANG Gong-she,SUN Shi-duo.Comparative detection of serum Leptin levels between obese and lean swine[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(6):5-8. |
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| Authors: | LONG Huo-sheng SUN Chao PANG Wei-jun YANG Gong-she SUN Shi-duo |
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| Institution: | (College of Animal Science and Technology,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | To investigate the expressional differences of ob gene between obese and lean swine,Western blotting and software Bandscan were employed to analyze the serum Leptin levels of Neijiang Pig,Bamei Pig,Landrace and Duroc.We found that Leptin resistance lies in obese swine;the serum Leptin level in obese swine is 323% higher than that in lean swine;a new peptide of about 33 ku in swine serum,which might be dimer of Leptin too,is structural homologous to Leptin.The fatness differences between obese and lean swine may be due to the pattern of ob gene expression. |
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| Keywords: | obese swine lean swine Leptin SDS-PAGE Western blotting |
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