A sensitive biosensor based on gold nanoparticles to detect <Emphasis Type="Italic">Ralstonia solanacearum</Emphasis> in soil |
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| Authors: | Salar Khaledian Maryam Nikkhah Masoud Shams-bakhsh Saeed Hoseinzadeh |
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| Institution: | 1.Department of Plant Pathology, Faculty of Agriculture,Tarbiat Modares University,Tehran,Iran;2.Department of Nanobiotechnology, Faculty of Biological Sciences,Tarbiat Modares University,Tehran,Iran |
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| Abstract: | Ralstonia solanacearum, the devastating causal agent of potato bacterial wilt, is a soil-borne bacterium that can survive in the soil for a long time. The development of sensitive on-field detection methods for this pathogen is highly desirable due to its widespread host range and distribution. A novel nanobiosensor was thus developed to detect unamplified genomic DNA of R. solanacearum in farm soil. Gold nanoparticles functionalized with single-stranded oligonucleotides served as a probe to detect R. solanacearum genomic DNA. The advantages of this strategy include rapidity, facile usage and being a visual colorimetric method. |
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