Node inoculation: A quick and easy technique to screen pigeonpea for resistance to Phytophthora blight |
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| Authors: | R Chand Chandra Kant Singh V Singh J Singh M Sharma |
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| Institution: | 1.Department of Mycology and Plant Pathology,Banaras Hindu University,Varanasi,India;2.International Crops Research Institute for the Semi-Arid Tropics,Hyderabad,India |
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| Abstract: | The petiole on pigeonpea was removed for easy, precise inoculation of node with Phytophthora drechsleri f. sp. cajani. After node inoculation, 96.0% plants were infected compared with 89.0% after stem-cut inoculation. Among various nodes inoculated on 30-day-old plants, the 5th node had the greatest relative susceptibility (90.0%), followed by the 3rd node (78.0%). This technique was validated on different cultivars (ICP 7119, Bahar, MA 6 and MAL 13), and 586 lines were successfully screened in the field, confirming the rapidity and effectiveness of the technique for resistance screening. |
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