| 高效液相色谱-蒸发光散射检测法测定茶叶中单糖和双糖 |
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| 引用本文： | 袁勇,黄建安,李银花,陈金华,袁玲,刘仲华.高效液相色谱-蒸发光散射检测法测定茶叶中单糖和双糖[J].茶叶科学,2010,30(6). |
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| 作者姓名： | 袁勇 黄建安 李银花 陈金华 袁玲 刘仲华 |
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| 作者单位： | 湖南农业大学茶学教育部重点实验室,国家植物功能成分利用工程技术研究中心,湖南,长沙,410128 |
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| 基金项目： | 湖南省重大科技专项“茯茶产业化关键技术研究与示范”(2008FJ1005); “十一五”农业部行业支撑计划“名优绿茶高效栽培加工关键技术研究”(NYHYZX07-021-12) |
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| 摘 要： | 建立了高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定茶叶中单糖和双糖的检测方法。采用ShodexNH2P-50-4E(4.6mm×250mm,5μm)色谱柱,以水和乙腈为流动相进行梯度洗脱,梯度洗脱程序为:0～14min,85%乙腈;14～16min,85%～70%乙腈;16～28min,70%乙腈;28～32min,70%～85%乙腈,流速:1.0mL/min,柱温:20℃,雾化管温度为30℃,漂移管温度为70℃,氮气压力为310.275Pa。鼠李糖、阿拉伯糖、木糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖在3.202～184.828μg范围内程良好的线性关系,8种糖的加标回收率在90.97%～105.07%之间,最低检测限(S/N=3)范围在6.976～1376.297ng。该方法具有操作简便,分离效果好等特点。
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| 关 键 词： | 高效液相色谱 蒸发光散射检测器 茶叶 单糖 双糖 |
Determination of Monosaccharides and Disaccharides in Tea by High Performance Liquid Chromatography-Evaporative Light Scattering Detector |
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| YUAN Yong,HUANG Jian-an,LI Yin-hua,CHEN Jin-hua,YUAN Ling,LIU Zhong-hua.Determination of Monosaccharides and Disaccharides in Tea by High Performance Liquid Chromatography-Evaporative Light Scattering Detector[J].Journal of Tea Science,2010,30(6). |
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| Authors: | YUAN Yong HUANG Jian-an LI Yin-hua CHEN Jin-hua YUAN Ling LIU Zhong-hua |
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| Institution: | YUAN Yong,HUANG Jian-an,LI Yin-hua,CHEN Jin-hua,YUAN Ling,LIU Zhong-hua (Key Lab of Tea Science of Ministry of Education,Hunan Agricultural University,National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanical,Changsha 410128,China) |
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| Abstract: | A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH2P-50-4E(4.6 mm×250 mm,5μm) column was used at 20℃,acetonitrile and water used as mobile phase,for gradient elusion with the flow rate of 1.0 mL/min.The ratio of organic solvent was controlled by a gradient elution procedure.The procedure was as follows:85%acetonitrile in 0～14 min,70%～80%acetonitrile 14～16 min,70%acetonitrile 16～28 min,then 70%～80%acetonitrile 28～32 min.At last,the ratio of acetonitrile increased to 85%. With the use of evaporative light-scattering detector,the nebulization chamber temperature was 30℃,the drift tube temperature was 70℃and nitrogen pressure was 310.275 Pa.The calibration curves of rhamnose,arabinose,xylose,fructose,mannose,glucose,sucrose and maltose showed good linearity in the range of 3.202～184.828μg.The recoveries of fortified 8 kinds of sugar were 90.97%～105.07%,limit of detention was 6.976～1 376.297 ng.Results indicated that the method was simple,rapid and could effectively separate the target component. |
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| Keywords: | HPLC ELSD tea monosaccharides disaccharides |
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