| 盐碱胁迫冬油菜的主导因素分析 |
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| 引用本文： | 许耀照,孙万仓,曾秀存,李彩霞,周喜旺.盐碱胁迫冬油菜的主导因素分析[J].草业科学,2013,30(3):423-429. |
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| 作者姓名： | 许耀照 孙万仓 曾秀存 李彩霞 周喜旺 |
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| 作者单位： | 河西学院,甘肃张掖,734000;甘肃农业大学,甘肃兰州,730070;河西学院,甘肃张掖734000;甘肃农业大学,甘肃兰州730070;天水市农业科学研究所,甘肃天水,741001 |
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| 基金项目： | 国家科技支撑计划项目，科技部农业科技成果转化资金项目，国家"12.5"863课题"强优势油菜杂交种的创制与应用"，农业部公益性行业(农业)科研专项经费项目，现代农业产业技术体系建设专项，河西学院校长科研基金项目计划 |
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| 摘 要： | 用4种盐分为A因素（NaCl、Na2SO4、Na2CO3和NaHCO3的依次盐分组成摩尔比为A1（1∶9∶9∶1）、A2（1∶1∶1∶1）、A3（9∶1∶1∶9）和A4（1∶1∶9∶9）与5种盐浓度为B因素（盐浓度B1、B2、B3、B4和B5 分别为10、20、30、40和50 mmol·L-1）的双因素试验，模拟20种不同盐度和pH的盐碱条件处理冬油菜“陇油6号”（Brassica campestris cv.Longyou No.6）的种子和幼苗7 d后，测定其种子发芽率、幼苗叶片细胞膜透性、丙二醛、脯氨酸、可溶性蛋白含量和根系活力等指标。结果表明，随着处理盐浓度的增加，种子发芽率、幼苗根系活力和可溶性蛋白含量降低，叶片细胞膜透性和脯氨酸的含量增加；降低和增加程度由A1～A4大体增强，不同盐组成处理间差异显著（P＜0.05）。用盐度(Na+)、pH值、CO32-和Cl-代表盐碱混合条件所有胁迫因素，胁迫因素与胁变指标间均具有高度线性相关性。pH值、盐度(Na+)和CO32-是盐碱混合条件对冬油菜胁迫的主导因素，而Cl-的胁迫作用也不可忽略。
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| 关 键 词： | 冬油菜 盐碱胁迫 盐度 pH 胁迫因素 胁迫指标 |
Analysis of the key stress factors in winter rape under simulated salinity-alkalinity mixed condition |
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| XU Yao zhao,SUN Wan cang,ZENG Xiu cun,LI Cai xia,ZHOU Xi wang.Analysis of the key stress factors in winter rape under simulated salinity-alkalinity mixed condition[J].Pratacultural Science,2013,30(3):423-429. |
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| Authors: | XU Yao zhao SUN Wan cang ZENG Xiu cun LI Cai xia ZHOU Xi wang |
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| Abstract: | Strain index of seed germination rate、seedling leaf cell membrane permeability、MDA content、proline content、soluble protein content and root activity of Longyou No. 6 seeds and seedlings were determined between A factors of 4 kinds of salt for NaCl, Na2SO4, Na2CO3 and NaHCO3, composed of salt mole ratio for A1 (1∶9∶9∶1), A2(1∶1∶1∶1), A3(9∶1∶1∶9) and A4(1∶1∶9∶9) and B factors of 5 kinds of salt concentration for B1 (10 mmol·L-1), B2 (20 mmol·L-1), B3 (30 mmol·L-1), B4 (40 mmol·L-1)and B5 (50 mmol·L-1) of the double factors. Twenty kinds of salinity and different pH of saline conditions were simulated to treat winter rape No.6 seeds and seedling 7D. The results showed that 20 kinds of salinity were from 10 to 50 mmol·L-1 and pH was between 8.50 and 10.24. Seed germination rate, seedling root activity and soluble protein content were decreased and leaf cell membrane permeability, MDA content and proline content were increased with the increasing of the concentration of salt treatment. The degree of increase and reduction became higher among A1 to A4 in turn. The different salt composition in treatment reached significant difference (P<0.05). All stress effect factors under saline mixed conditions were caused by salinity (Na+), pH, CO32- and Cl-. Stress factors of saline alkali and strain index of Longyou No. 6 seeds and seedlings had high linear correlation. The dominant factors of winter rape stress were pH、salinity (Na+)、CO32- and stress effect on winter rape Longyou No. 6 of Cl- could not be ignored. |
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| Keywords: | winter rape salinity alkalinity stress salinity pH stress factors stress index |
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