| 5个苜蓿品种叶片表面蜡质覆盖与抗旱性的关系 |
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| 引用本文： | 周玲艳,刘胜洪,秦华明,黎佩凤,杨凤婷,赵钢.5个苜蓿品种叶片表面蜡质覆盖与抗旱性的关系[J].草业科学,2013,30(4):596-601. |
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| 作者姓名： | 周玲艳 刘胜洪 秦华明 黎佩凤 杨凤婷 赵钢 |
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| 作者单位： | 1. 仲恺农业工程学院生命科学学院,广东广州,510225
2. 暨南大学理工学院,广东广州,510623 |
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| 基金项目： | 广东省自然基金，广东省科技计划 |
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| 摘 要： | 以5个苜蓿（Medicago sativa）品种苜蓿王、阿尔冈金、大富豪、WL525和WL2321为材料，通过扫描电镜观察叶片表面蜡质覆盖情况，以热氯仿法抽提和测定叶片表面蜡质含量，并测定叶片叶绿素浸提率和脯氨酸含量，同时以质量分数为20% 的PEG 6000进行模拟干旱处理，分析叶片表面蜡质覆盖量与苜蓿抗旱性之间的关系。结果表明，苜蓿王的叶片表面蜡质分布较密集，含量较高，而WL525叶片表面蜡质分布较稀疏，含量较少；苜蓿王的叶片叶绿素浸提速率相对较慢，WL525的叶片叶绿素浸提速率相对较快；苜蓿王的脯氨酸含量较高，而WL525的脯氨酸含量较低；模拟干旱处理发现苜蓿王对干旱敏感性最低。综合考虑，苜蓿品种叶片表面蜡质覆盖量与其抗旱能力呈正相关。
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| 关 键 词： | 苜蓿 叶片表面蜡质 抗旱性 |
A study on relationship between leaf epicuticular wax coverage and drought resistance of five alfalfa varieties |
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| ZHOU Ling yan,LIU Sheng hong,QIN Hua ming,LI Pei feng,YANG Feng ting,ZHAO Gang.A study on relationship between leaf epicuticular wax coverage and drought resistance of five alfalfa varieties[J].Pratacultural Science,2013,30(4):596-601. |
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| Authors: | ZHOU Ling yan LIU Sheng hong QIN Hua ming LI Pei feng YANG Feng ting ZHAO Gang |
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| Abstract: | Cuticular wax, exposed at the outermost surface of plant organs, plays an important role in interactions of plants and environment， and also plays a critical role in plant drought tolerance by reducing cuticular water loss. In this study, five alfalfa (Medicago sativa) cultivars: Alfaking, Algonquin, Millionaire, WL525 and WL2321 were used as testing materials to analyze the relationship between leaf epicuticular wax coverage and drought resistance, by observing leaf surface wax coverage and determining wax content, chlorophyll extraction rate and proline content. The results showed that among the five alfalfa varieties, the leaf epicuticular wax distribution of Alfaking was more intensive and wax content was higher, while the leaf epicuticular wax distribution of WL525 was sparse and wax content was lower. The chlorophyll extraction rate of Alfaking was relatively slow, however, that of the WL525 was relatively fast. The proline content of Alfaking was the highest, but that of WL525 was minimum. The drought sensitivity of Alfaking was the weakest when they were treated with 20% PEG 6000 (the simulated drought treatment). To comprehensively considered, the leaf epicuticular wax coverage of alfalfa has correlation with drought resistance. |
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| Keywords: | alfalfa epicuticular wax drought resistance |
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