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| 猪肉和牛奶中10种磺胺类药物残留检测超高效液相色谱法研究 | |
| 引用本文： | 胡海燕.猪肉和牛奶中10种磺胺类药物残留检测超高效液相色谱法研究[J].中国兽药杂志,2009,43(8):1-4. |
| 作者姓名： | 胡海燕 |
| 作者单位： | 1. 中国兽医药品监察所,北京,100081 2. 中国药科大学,南京,211198 3. 沃特世科技有限公司北京分公司,北京,100025 |
| 摘 要： | 建立了猪肉和牛奶中磺胺嘧啶（SD）、磺胺噻唑（ST）、磺胺二甲嘧啶（SM2）、磺胺甲氧嗪（SMP）、磺胺氯哒嗪（SPD）、磺胺间甲氧嘧啶（SMM）、磺胺甲基异嗯唑（SM2）、磺胺氯吡嗪（Esb3）、磺胺地索辛（SDM）和磺胺喹嗯啉（SQ）共10种磺胺类药物残留检测的超高效液相色谱（UPLC）法。色谱条件：色谱柱为Acquity UPLC BEH C18柱（2．1mm×50mm，1．7μm）；流动相为50％甲醇乙腈溶液-2％乙酸水溶液，梯度洗脱；紫外检测波长270nm；柱温30℃；进样量4μL；外标法定量。结果表明：10种组分在20～1000ng／mL浓度范围内，呈良好线性关系，相关系数R^2均大于0．998；方法检出限为20ng／g，定量限为50ng／g；在猪肉和牛奶中添加浓度分别为50、100、200ng／g时，平均回收率为75．1％～99．8％，批内、批间平均RSD均小于13．2％。 |
| 关 键 词： | 猪肉 牛奶 磺胺类药物 残留 UPLC |
| 收稿时间： | 7/8/2009 12:00:00 AM |
| 修稿时间： | 7/8/2009 12:00:00 AM |
Simultaneous Determination of Ten Sulfonamides Residues in Pig Muscle and Milk by UPLC |
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| huhaiyan.Simultaneous Determination of Ten Sulfonamides Residues in Pig Muscle and Milk by UPLC[J].Chinese Journal of Veterinary Drug,2009,43(8):1-4. | |
| Authors: | huhaiyan |
| Institution: | China Institute of Veterinary Drug Control |
| Abstract: | A UPLC method was established for ten sulfonamides including sulfadiazine （SD）, sulfathiazole （ST）, sulfadimidine（ SM2 ）, sulfamethoxypyridazine （SMP） , sulfachlorpyridazine （ SPD）, sulfamonomethoxine （ SMM ）, sulfamethoxazole （SMZ）, sulfachloropyrazine （ Esb3 ）, sulfadimethoxine （SDM） and sulfaquinoxaline （SQ） in pig muscle and milk. UPLC conditions：the chromatography column was Acquity UPLC BEH Cls column of 2.1 mm × 50 mm, 1. 7 μm;mobile phase was acetonitrile - methanol（ 1 ： 1, V/V） - water（2% acetic acid） ; Column temperature was 30 ℃ ; Determination was by UV detection at 270 nm; Injection volume is 4 μL. The calibration curve was good linear between the peak areas and concentrations of sulfonamides from 20 to 1 000 ng/mL, the correlation coefficient R^2 〉 0.998. The limit of detection was 20 ng/g; The limit of quantification was 50 ng/g. The average recoveries from spiked pig muscle and milk at the three concentrations of 50, 100 and 200 ng/g ranged from 75.1% -99.8% ,and intra- day and interday（n=5） batch variation coefficients were less than 13.2%. |
| Keywords: | UPLC |
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