| 山羊基因组文库的构建研究 |
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| 引用本文： | 唐 爽,安志兴,张明烽,宋红卫,张 涌.山羊基因组文库的构建研究[J].中国农学通报,2005,21(10):19-19. |
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| 作者姓名： | 唐 爽 安志兴 张明烽 宋红卫 张 涌 |
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| 作者单位： | 西北农林科技大学生物工程研究所,陕西,杨陵,712100 |
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| 基金项目： | 国家奶业重大科技专项(2002BA518A01-2)。 |
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| 摘 要： | 构建了山羊基因组文库，为筛选山羊乳蛋白基因，构建山羊乳蛋白基因定点整合的转基因敲入载体奠定基础。全血提取基因组DNA，Sau3AΙ部分酶切，分级分离回收15~23 kb片段，将片段插入Lambda BlueSTAR BamHΙ vector，连接并包装后，NotI酶切鉴定。结果表明，文库滴度为3.4×106；共得到1.7×106个有效克隆，插入片断长度在15~23kb范围，成功构建了西农萨能奶山羊基因组文库。
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| 关 键 词： | 基因组文库 西农萨能奶山羊 |
| 收稿时间： | 2005-06-02 |
| 修稿时间： | 2005-06-022005-06-20 |
Construction of Goat Genomic Library |
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| Tang Shuang,An Zhixing,Zhang Mingfeng,Song Hongwei,Zhang Yong.Construction of Goat Genomic Library[J].Chinese Agricultural Science Bulletin,2005,21(10):19-19. |
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| Authors: | Tang Shuang An Zhixing Zhang Mingfeng Song Hongwei Zhang Yong |
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| Institution: | (Institute of Bio-engineering, Northwest A & F University, Yangling, 712100, Yangling Shaanxi 712100) |
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| Abstract: | Goat genomic DNA library was constructed for screening milk-protein-encoding gene of goat and construction of knock-in vector. Genomic DNA was prepared from blood sample, partially digested with Sau3AΙ.15！23kb fragment was fractionated and ligated to Lambda BlueSTAR BamHΙ Arms. Titer of the library is 3.4×106; 1.7×106 clones were obtained with inserts size ranging from 15~23kb. |
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| Keywords: | Genomic library Xinong saanen goat |
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