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| 东亚钳蝎神经毒素基因BmKCT的克隆与表达 | |
| 引用本文： | 张守涛,郭蔼光,肖乐义.东亚钳蝎神经毒素基因BmKCT的克隆与表达[J].西北农林科技大学学报(社会科学版),2002,30(6):30-33. |
| 作者姓名： | 张守涛 郭蔼光 肖乐义 |
| 作者单位： | 1. 河南省科学院,生物技术开发中心,河南,郑州,450002;西北农林科技大学,生命科学学院,陕西,杨陵,712100 2. 西北农林科技大学,生命科学学院,陕西,杨陵,712100 3. 河南省科学院,生物技术开发中心,河南,郑州,450002 4. 郑州大学医学院,河南郑州,450002 |
| 基金项目： | 河南省重点科技攻关项目(0223032300) |
| 摘 要： | 从蝎毒腺中提取总RNA,通过RT-PCR得到东亚钳蝎神经毒素BmKCT的cDNA,将其连接到pUCm-T载体中。序列分析表明,该基因开放阅读框架编码59个氨基酸残基,其中24个为信号肽,其余35个为成熟肽,与Genebank上登录的相同。将蝎神经毒素cDNA再经PCR扩增除去信号肽序列,克隆到pTrcHisA表达载体中,转化大肠杆菌BL21(DE3),经IPTG诱导可高效表达分子质量为10ku左右的融合蛋白。表达产物约占菌体总蛋白的25%。 |
| 关 键 词： | 蝎毒素 cDNA 融合表达 |
| 文章编号： | 1671-9387(2002)06-0030-04 |
| 收稿时间： | 2002/6/17 0:00:00 |
| 修稿时间： | 2002年6月17日 |
Cloning and expression of a neurotoxin from chinese scorpion Buthus martensii Karsch in Escherichia coli |
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| ZHANG Shou tao,,GUO Ai guang,XIAO Le yi,DONG Jian jun,ZHAO Tian zeng,XUE Qi geng,KONG Tian han,GUO Wei.Cloning and expression of a neurotoxin from chinese scorpion Buthus martensii Karsch in Escherichia coli[J].Journal of Northwest Sci-Tech Univ of Agr and,2002,30(6):30-33. | |
| Authors: | ZHANG Shou tao GUO Ai guang XIAO Le yi DONG Jian jun ZHAO Tian zeng XUE Qi geng KONG Tian han GUO Wei |
| Institution: | 1(1 Biotechnology Developing Center of Henan Academy of Sciences,Zhengzhou,Henan 450002,China;2 College of Life Science,Northwest Sci Tech University of Agriculture and Forest,Yangling,Shaanxi 712100,China;3 College of Medical,Zhengzhou University,Zhengzhou,Henan 450002,China) |
| Abstract: | A neurotoxin cDNA was cloned from Chinese scorpion Buthus martensii Karsch by RT-PCR.The cDNA was cloned into the pUCm-T vector and sequenced.It has a ORF encoding 59 amino acid residues and a 24 residues signal peptide.The cDNA encoding the mature peptide was amplified by PCR and was cloned into pTrcHisA vector.The recombinant vector was transformed into E.coli BL21(DE3).The E.coli highly expressed the fusion protein whose mollecular weight is 10 ku,after induced by 0.1 mol/L IPTG.The expressed protein was accumulated up to more than 25% of total bacterial protein. |
| Keywords: | scorpion toxin cDNA fusion expression |
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