| [鱼师]诺卡菌特异性PCR快速检测方法的建立 |
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| 引用本文： | 蒋依依,李安兴.[鱼师]诺卡菌特异性PCR快速检测方法的建立[J].南方水产,2011,7(6):47-51. |
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| 作者姓名： | 蒋依依 李安兴 |
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| 作者单位： | 中山大学生命科学学院,水产品安全教育部重点实验室,有害生物控制与资源利用国家重点实验室,广东广州510275 |
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| 基金项目： | 广东省省级财政鱼病防治专项“广东省养殖鱼类诺卡氏菌病的流行病学和综合防治研究” |
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| 摘 要： | 鱼师]诺卡菌（Nocardia seriolae）是危害中国南方大口黑鲈（Micropterus salmoides）养殖业的主要病原之一，由于该菌在培养基上生长缓慢，对其分离鉴定造成诸多不便。文章根据鱼师]诺卡菌16S-23S转录间隔区（ITS）序列设计特异性引物建立了鱼师]诺卡菌特异性PCR快速检测方法。试验结果表明，利用设计的特异性PCR引物只能扩增出鱼师]诺卡菌特异性片段，检出限为5pg模板DNA。在此基础上研制了PCR快速检测试剂盒并对鱼师]诺卡菌人工感染的大口黑鲈组织进行了检测，结果显示该试剂盒能从未出现明显发病症状的大口黑鲈组织中检出阳性片段，阳性率为100％，比传统细菌分离鉴定方法更加灵敏、快速且高效，具有较好的应用前景。
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| 关 键 词： | [鱼师]诺卡菌 ITS 聚合酶链式反应 检测 大口黑鲈 |
Establishment of a specific PCR assay to detect Nocardia seriolae |
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| JIANG Yiyi,LI Anxing.Establishment of a specific PCR assay to detect Nocardia seriolae[J].South China Fisheries Science,2011,7(6):47-51. |
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| Authors: | JIANG Yiyi LI Anxing |
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| Institution: | JIANG Yiyi,LI Anxing(Key Lab.for Aquatic Food Products Safety of Ministry of Education,State Key Lab.of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China) |
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| Abstract: | Nocardia seriolae,one of the main pathogenic bacterium of cultured largemouth bass Micropterus salmoides in South China,grows very slowly in culture medium,so it is difficult for its isolation and identification.In present study,two specific primers were designed according to genomic sequence of 16S~23S intergenic spacers(ITS) to establish a rapid specific PCR assay to detect N.seriolae.Results indicate that the 2 primers are very specific to detect N.seriolae and show positive to very little of DNA amount ... |
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| Keywords: | Nocardia seriolae ITS polymerase chain reaction detection Micropterus salmoides |
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