| 可降解水体中烟嘧磺隆微生物的分离与筛选 |
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| 引用本文： | 杨亚君,刘顺,武丽芬,庞民好,陶哺,张金林.可降解水体中烟嘧磺隆微生物的分离与筛选[J].农药学学报,2007,9(3):275-279. |
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| 作者姓名： | 杨亚君 刘顺 武丽芬 庞民好 陶哺 张金林 |
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| 作者单位： | 1.河北农业大学 植物保护学院, 河北 保定 071001 |
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| 基金项目： | 河北农业大学校科研和教改项目 |
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| 摘 要： | 为了筛选出可降解烟嘧磺隆的微生物,利用富集培养技术从水样中分离得到了5株能以烟嘧磺隆为唯一氮源、碳源和能源的微生物,分别编号为YF1、YB1、YB2、YB3和YB4,其中YF1为真菌,其余均为细菌。将所得菌株定量接种于含不同浓度烟嘧磺隆的培养基中,于30℃、150 r/min摇床上振荡培养5 d后,利用HPLC检测烟嘧磺隆含量,计算降解率。结果发现:这些菌株对低浓度烟嘧磺隆的降解率显著高于对高浓度烟嘧磺隆的降解率,当培养基中烟嘧磺隆的含量为2 mg/kg 时,YF1对该药剂的降解率最高,为80.31 %,其次是YB1和YB2,降解率分别为 78.18% 和73.72%,而YB3和YB4对药剂的降解率只有36.82 %和25.75 %。对3株降解率较高的菌株进行了初步鉴定,发现YF1为黑曲霉Aspergillus niger,YB1和YB2均为芽孢杆菌Bacillus sp.。
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| 关 键 词： | 烟嘧磺隆 微生物降解 富集培养 |
| 文章编号： | 1008-7303（2007）03-0275-05 |
| 收稿时间： | 2007/4/10 0:00:00 |
| 修稿时间： | 2007-04-10 |
Isolation and Screening of Microorganisms Capableof Degrading Nicosulfuron in Water |
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| YANG Ya-jun,LIU Shun,WU Li-fen,PANG Min-hao,TAO Bu and ZHANG Jin-lin.Isolation and Screening of Microorganisms Capableof Degrading Nicosulfuron in Water[J].Chinese Journal of Pesticide Science,2007,9(3):275-279. |
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| Authors: | YANG Ya-jun LIU Shun WU Li-fen PANG Min-hao TAO Bu and ZHANG Jin-lin |
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| Institution: | 1.College of Plant Protection,Hebei Agricultural University,Baoding 071001,Hebei Province,China2.Hebei Institute for the Control of Agrochemicals,Shijiazhuang 050031,China |
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| Abstract: | In order to obtain the microorganisms degrading nicosulfuron,five strains of microorganisms numbered as YB1,YB2,YB3,YB4 (bacteria),and YF1 (fungi) were isolated by enrichment culture with nicosulfuron as the only source of carbon,nitrogen and energy.All of the microorganisms were cultured in culture medium containing different concentrations of nicosulfuron,then put it in swing bed at 30℃ and shook at 150 r/min for 5 days,detected the concentration of nicosulfuron by HPLC and calculated the degradation efficiency.It was shown that the degradation rates of the five strains of microorganisms were higher in low concentration than in high concentration of nicosulfuron,with YF1 having the highest degradation rate of 80.31％,secondly,YB1 and YB2 having the degradation rate of 78.18％ and 73.72％,respectively,however,YB3 and YB4 having lower degradation rate of 36.82％ and 25.75％,respectively.It was also discovered that YF1 was Aspergillus niger,YB1 and YB2 were Bacillus sp.by primary identification. |
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| Keywords: | nicosulfuron microbial degradation enrichment culturing |
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