| 灵昆鸡群体微卫星DNA遗传多样性研究 |
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| 引用本文： | 袁青妍,虞德兵,吴春琴,李国勤,王德前,卢立志.灵昆鸡群体微卫星DNA遗传多样性研究[J].浙江农业学报,2010,22(4):0. |
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| 作者姓名： | 袁青妍 虞德兵 吴春琴 李国勤 王德前 卢立志 |
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| 作者单位： | 1.浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310021；;2.南京农业大学 动物科学技术学院,江苏 南京210095；;3.温州市农业科学研究院 动物研究所,浙江 温州 325006 |
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| 基金项目： | 浙江省重大科技攻关项目 |
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| 摘 要： | 为阐明灵昆鸡群体遗传变异和遗传结构状况,采用23个家鸡特异性的微卫星标记对该鸡种自然群体中30个个体进行PCR扩增和聚丙烯酰胺凝胶电泳分析,检测其等位基因。结果表明,共检测到174个等位基因,23个座位都呈现出多态性,23个微卫星座位的等位基因数在3～12个,平均等位基因数为7.5个。群体平均杂合度和平均多态信息含量分别为0.758和0.723。分析结果表明灵昆鸡自然群体遗传多样性较丰富。
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| 关 键 词： | 微卫星标记 灵昆鸡 遗传多样性 杂合度 多态信息含量 |
Genetic diversity of Lingkun chicken based on microsatellite markers |
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| YUAN Qing-yan,YU De-bing,WU Chun-qin,LI Guo-qin,WANG De-qian,LU Li-zhi.Genetic diversity of Lingkun chicken based on microsatellite markers[J].Acta Agriculturae Zhejiangensis,2010,22(4):0. |
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| Authors: | YUAN Qing-yan YU De-bing WU Chun-qin LI Guo-qin WANG De-qian LU Li-zhi |
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| Institution: | 1Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2 College of Animal Science & Technique, Nanjing Agriculture University, Nanjing 210095, China; 3 Institute of Animal Science, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China) |
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| Abstract: | In order to assess Lingkun chicken's genetic variation and population structure, 23 chicken-specific microsatellite loci were used to analyze the alleles for 30 individuals from natural population using PCR and polyacrylamide gel electrophoresis. A total of 174 alleles were detected across the 23 microsatellite loci. All the loci were polymorphic and the number of alleles varied from 3 to 12, and the mean number was 7.5 alleles per locus. The average heterozygosity and polymorphism information content of 23 microsatellite loci were 0.758 and 0.723, respectively. The results indicated that there was high genetic diversity in the natural population of Lingkun chicken． |
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| Keywords: | microsatellite marker Lingkun chicken genetic diversity heterozygosity polymorphism information content |
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