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| 花生二氢黄酮醇还原酶基因(DFR)的克隆及表达分析 | |
| 引用本文： | 郭凤丹,夏晗,袁美,王兴军.花生二氢黄酮醇还原酶基因(DFR)的克隆及表达分析[J].农业生物技术学报,2011,19(5). |
| 作者姓名： | 郭凤丹 夏晗 袁美 王兴军 |
| 作者单位： | 1. 山东师范大学生命科学院,济南,250014;山东省农业科学院高新技术研究中心,济南,250100 2. 山东省农业科学院高新技术研究中心,济南,250100 3. 山东省花生研究所,青岛,266100 |
| 基金项目： | 国家自然科学基金(No.30871324,No.31000720); 山东省优秀中青年科学家奖励基金(No.2006BS06008); 山东省自然科学基金(No.Y2008D44); 山东省“泰山学者”专项基金共同资助 |
| 摘 要： | 二氢黄酮醇还原酶(dihydroflavonol 4-reductase,DFR)是植物花青素合成过程中的关键酶,催化二氢黄酮醇生成无色花青素。本研究利用花生(Arachis hypogae a L.)未成熟种子cDNA文库,通过大规模EST测序,从花生中克隆了DFR基因的全长cDNA序列。序列分析表明,花生DFR蛋白氨基酸序列与其他植物来源的DFR有很高的同源性。利用花生cDNA芯片和半定量RT-PCR方法对花生DFR基因表达模式的研究发现,DFR基因在果针中表达水平最高,其次是花,在根和叶中有微量表达;不同种皮颜色花生品种的种子中表达差异明显,随种皮颜色的加深,DFR基因表达增强,在中花9号黑色种子中表达量最高。对茎叶紫色花生种质材料的研究表明,在紫色组织中DFR基因表达较强。结果表明DFR表达量与花青素积累量呈正相关,说明DFR催化反应是花青素合成途径的关键步骤。 |
| 关 键 词： | 花生 cDNA文库 花青素 DFR 基因表达 |
Cloning of Dihydroflavonol 4-reductase Gene (DFR) from Peanut (Arachis hypogaea L.) and Its Expression Analysis |
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| Guo Fengdan,Xia Han,Yuan Mei,Wang Xingjun.Cloning of Dihydroflavonol 4-reductase Gene (DFR) from Peanut (Arachis hypogaea L.) and Its Expression Analysis[J].Journal of Agricultural Biotechnology,2011,19(5). | |
| Authors: | Guo Fengdan Xia Han Yuan Mei Wang Xingjun |
| Institution: | Guo Fengdan1,2 Xia Han2 Yuan Mei3 Wang Xingjun1,2* 1 College of Life Sciences,Shandong Normal University,Jinan 250014,China,2 High-tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,3 Shandong Peanut Research Institute,Qingdao 266100 |
| Abstract: | Dihydroflavonol 4-reductase (DFR) is a key enzyme converting dihydroflavonols to leucoanthocyanidins in the biosynthesis of anthocyanins. EST sequencing was carried out using a cDNA library of peanut(Arachis hypogaea L.) immature seed and full length cDNA of peanut DFR was cloned. Sequence alignment showed that DFR was highly conserved among different plant species. Peanut cDNA microarray and semi-quantitative RT-PCR were used to analysis the expression of DFR in different tissues. Results indicated that th... |
| Keywords: | Arachis hypogaea L cDNA library Anthocyanin DFR Gene expression |
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