| 水稻单倍体诱导基因Os MATL突变体的创制与分析 |
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| 引用本文： | 文钦,贾思思,王加峰,黄翠红,王慧,陈志强,郭涛.水稻单倍体诱导基因Os MATL突变体的创制与分析[J].作物学报,2021(5):827-836. |
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| 作者姓名： | 文钦 贾思思 王加峰 黄翠红 王慧 陈志强 郭涛 |
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| 作者单位： | 华南农业大学/国家植物航天育种工程技术研究中心 |
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| 基金项目： | 广东省现代种业重大专项(2018B020206002);国家现代农业产业技术体系建设专项(CARS-01-17)资助。 |
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| 摘 要： | MTL基因是玉米中控制单倍体诱导性状的关键基因,该基因在作物中的功能高度保守。OsMATL是MTL在水稻中的同源基因,已有研究证实该基因的突变可以诱导水稻单倍体产生,但不同遗传背景下,OsMATL基因的突变效应有待明确。本研究以日本晴(粳稻)和华航48(籼稻)为材料,利用CRISPR/Cas9技术对OsMATL基因启动子区和编码区的不同位点进行编辑,成功获得OsMATL基因不同遗传背景系列突变体。分析了不同突变位点对结实率的影响,发现OsMATL基因编码区突变后结实率为2%~15%,且存在不同类型的败育籽粒,而启动子区域的大片段缺失对结实率没有显著影响;此外,籼稻背景突变体的结实率高于粳稻突变体。本研究创制的不同类型OsMATL突变体为进一步研究水稻孤雌生殖单倍体诱导的机制提供了基础材料。
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| 关 键 词： | 水稻 单倍体诱导基因 突变体 |
Construction and identification of haploid induction gene OsMATL mutants in rice |
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| WEN Qin,JIA Si-Si,WANG Jia-Feng,HUANG Cui-Hong,WANG Hui,CHEN Zhi-Qiang,GUO Tao.Construction and identification of haploid induction gene OsMATL mutants in rice[J].Acta Agronomica Sinica,2021(5):827-836. |
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| Authors: | WEN Qin JIA Si-Si WANG Jia-Feng HUANG Cui-Hong WANG Hui CHEN Zhi-Qiang GUO Tao |
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| Institution: | (National Engineering Research Center of Plant Space Breeding,South China Agricultural University,Guangzhou 510642,Guangdong,China Abstract) |
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| Abstract: | MTL is the key gene for control haploid induction traits in maize,which is highly function conserved in crops.Os MATL is a homologous gene of MTL in rice.It has been confirmed that Os MATL mutation can induce haploid in rice,but the effect of Os MATL mutation in different genetic backgrounds remains to be determined.In this study,Nipponbare(Oryza sativa japonica)and Huahang 48(Oryza sativa indica)were used as research materials.CRISPR/Cas9 technology was used to edit different sites in the promoter region and coding region of Os MATL,and a series of Os MATL mutations with different genetic backgrounds were successfully obtained.The effect of different mutation sites on seed setting rate was analyzed,and it was found that the seed setting rate was 2%–15%in the coding region of Os MATL.There were different types of abortion seeds,while large fragment deletion in the promoter region had no significant differences on seed setting rate.In addition,the background of indica rice mutants of seed setting rate was higher than japonica rice mutants.Os MATL mutants of different types created in this study provided basic materials for further research on the mechanism of parthenogenetic haploid induction in rice. |
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| Keywords: | rice haploid induction gene mutant |
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