| 甘蓝型油菜千粒重全基因组关联分析 |
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| 引用本文： | 张春,赵小珍,庞承珂,彭门路,王晓东,陈锋,张维,陈松,彭琦,易斌,孙程明,张洁夫,傅廷栋.甘蓝型油菜千粒重全基因组关联分析[J].作物学报,2021(4):650-659. |
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| 作者姓名： | 张春 赵小珍 庞承珂 彭门路 王晓东 陈锋 张维 陈松 彭琦 易斌 孙程明 张洁夫 傅廷栋 |
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| 作者单位： | 南京农业大学/作物遗传与种质创新国家重点实验室;江苏省农业科学院经济作物研究所/农业农村部长江下游棉花与油菜重点实验室/中国江苏省现代作物生产协同创新中心;华中农业大学植物科学技术学院/作物遗传改良国家重点实验室 |
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| 基金项目： | 国家重点研发计划项目(2018YFD0100602);国家现代农业产业技术体系建设专项(CARS-12);国家自然科学基金项目(32001581);江苏省农业科技自主创新基金(CX(19)3055);江苏省基础研究计划(自然科学基金)项目(BK20190260);中央高校基本科研业务费专项资金项目(2662016PY063)资助。 |
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| 摘 要： | 千粒重是油菜产量构成的重要因素之一。本研究利用高通量SNP芯片对496份具有代表性的油菜种质资源进行基因型分析,考察群体在3个环境(14NJ、15TZ、16TZ)中的千粒重表型,利用混合线性模型(mixed linear model,MLM)和一般线性模型(general linear model,GLM)进行全基因组关联分析。结果表明,本群体在3个环境中千粒重的广义遗传力为63.12%。MLM模型检测到6个显著位点,解释28.92%的表型变异;GLM模型检测到61个显著位点,解释47.08%的表型变异。合并共同位点后得到62个显著位点,联合解释47.31%的表型变异。这些位点分布在基因组所有染色体上,在A07、A03和C06染色体上分别检测到数目最多的9、8和7个位点。其中效应最大的位点Bn-scaff_17526_1-p1066214位于C09染色体,在MLM和GLM模型中表型贡献值分别为5.55%和15.26%。21个位点与前人报道的QTL重叠,其中8个位点得到至少2个群体的验证。其余41个位点为新鉴定的位点,其中多个位点效应高且在多环境中被检测到,如位点Bn-A03-p560769、Bn-scaff_15743_1-p599416和Bn-scaff_15743_1-p590955等。在11个位点附近找到DGAT、EOD3、AGL61、WRI1、DA2、RAV1等拟南芥已报道千粒重基因的同源基因。本研究结果有助于解析甘蓝型油菜千粒重的遗传基础,为研究千粒重的调控机制、指导千粒重的遗传改良奠定基础。
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| 关 键 词： | 甘蓝型油菜 千粒重 产量 关联分析 SNP标记 |
Genome-wide association study of 1000-seed weight in rapeseed(Brassica napus L.) |
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| ZHANG Chun,ZHAO Xiao-Zhen,PANG Cheng-Ke,PENG Men-Lu,WANG Xiao-Dong,CHEN Feng,ZHANG Wei,CHEN Song,PENG Qi,YI Bin,SUN Cheng-Ming,ZHANG Jie-Fu,FU Ting-Dong.Genome-wide association study of 1000-seed weight in rapeseed(Brassica napus L.)[J].Acta Agronomica Sinica,2021(4):650-659. |
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| Authors: | ZHANG Chun ZHAO Xiao-Zhen PANG Cheng-Ke PENG Men-Lu WANG Xiao-Dong CHEN Feng ZHANG Wei CHEN Song PENG Qi YI Bin SUN Cheng-Ming ZHANG Jie-Fu FU Ting-Dong |
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| Institution: | (Nanjing Agricultural University/State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,Jiangsu,China;Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed(Nanjing),Ministry of Agriculture and Rural Affairs/Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing 210014,Jiangsu,China;National Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China) |
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| Abstract: | Thousand-seed weight(TSW)is one of the important component of seed yield.In this study,a collection of 496 representative rapeseed accessions were genotyped by the high-throughput 60K SNP array for TSW in three environments(14NJ,15TZ,16TZ).The genome-wide association study(GWAS)of TSW was performed via the MLM(Mixed linear model)and GLM(General linear model).The results showed that the broad sense heritability of TSW was 63.12%in three environments.Six and 61 loci were detected with MLM and GLM,which explained 28.92%and 47.08%of the phenotypic variance,respectively.Combining the common loci between two models,62 significant loci were obtained and accounted for 47.31%of the phenotypic variance.These loci were distributed on all chromosomes of the genome,with the largest number of 9,8,and 7 loci detected on Chromosome A07,A03,and C06,respectively.The most significant locus Bn-scaff_17526_1-p1066214 was detected on C09,and accounted for 5.55%and 15.26%of the phenotypic variance in MLM and GLM,respectively.Among them,21 loci overlapped with previously reported QTLs,of which 8 loci were verified by at least two populations.The remaining 41 loci were newly detected,and many of them had high effects and were detected in multiple environments,such as Bn-A03-p560769,Bn-scaff_15743_1-p599416 and Bn-scaff_15743_1-p590955.Besides,11 candidates orthologous to documented Arabidopsis seed weight genes,like DGAT,EOD3,AGL61,WRI1,DA2,and RAV1,were found near our GWAS loci.The results are helpful for analyzing the genetic basis of TSW of rapeseed and lay a foundation for studying the regulation mechanism and guiding the genetic improvement of TSW. |
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| Keywords: | Brassica napus L 1000-seed weight yield GWAS SNP |
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