| 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析 |
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| 引用本文： | 王琰琰,王俊,刘国祥,钟秋,张华述,骆铮珍,陈志华,戴培刚,佟英,李媛,蒋勋,张兴伟,杨爱国.基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J].作物学报,2021(7):1259-1274. |
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| 作者姓名： | 王琰琰 王俊 刘国祥 钟秋 张华述 骆铮珍 陈志华 戴培刚 佟英 李媛 蒋勋 张兴伟 杨爱国 |
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| 作者单位： | 中国农业科学院烟草研究所;四川省烟草公司德阳市公司;四川省烟草科学研究所 |
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| 基金项目： | 中国烟草总公司四川省公司科技项目(SCYC201903);中国农业科学院科技创新工程(ASTIP-TRIC01);中央级公益性科研院所基本科研业务费专项(1610232019008);农作物种质资源保护与利用专项(2020NWB038);国家作物种质资源库(NCGRC-2020-18)资助。 |
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| 摘 要： | 为从分子水平研究我国雪茄烟种质资源的遗传多样性差异并建立雪茄烟品种的DNA指纹图谱数据库,本研究利用43对多态性好的SSR引物对220份雪茄烟种质进行遗传多样性分析,筛选出14对核心引物对雪茄烟种质进行指纹图谱的构建。结果表明,43对SSR引物在220份雪茄烟种质材料中共扩增出243个等位基因,平均每个标记5.65个,变幅为2~13,每个位点的多态性信息量(polymorphism information content,PIC)变化为0.2078~0.9087,平均为0.6360。有效等位基因数(number of effective alleles,Ne)范围为1.3081~11.7876,平均有效等位基因数为3.9077;观测杂合度(observed heterozygosity,Ho)变化范围为0.0828~0.7639,平均为0.3191;预期杂合度(expected heterozygosity,He)的变化范围为0.2361~0.9172,平均为0.6809;种群平均Shannon遗传多样性指数(Shannon genetic diversity index,I)为1.3756,遗传距离在0.0233~0.9286之间,平均遗传距离0.6816。聚类分析表明,在遗传距离为0.74处,可将供试雪茄烟资源分为3个类群。Structure群体遗传结构分析和主成分分析将所有的供试材料划分为2个类群。根据引物的分析和表型鉴定结果,确定良种、辅善和满耳朵,山东大叶和牡丹江05-1,Florida513和CA0701为异名同种,一个品种保留一份种质,剩余216份不同种质。从43对SSR引物中筛选出14对可区分所有供试材料的SSR引物作为核心引物构建了216个雪茄烟品种的指纹图谱。我国雪茄烟种质资源具有较高水平的遗传多样性,本研究构建的雪茄烟种质资源SSR指纹图谱库及遗传分析的结果在分子水平上为筛选、鉴定优质雪茄烟种质资源、挖掘重要基因以及拓宽雪茄烟遗传育种基础等工作提供科学依据。
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| 关 键 词： | 雪茄烟 种质资源 SSR标记 遗传多样性 指纹图谱 |
Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources |
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| WANG Yan-Yan,WANG Jun,LIU Guo-Xiang,ZHONG Qiu,ZHANG Hua-Shu,LUO Zheng-Zhen,CHEN Zhi-Hua,DAI Pei-Gang,TONG Ying,LI Yuan,JIANG Xun,ZHANG Xing-Wei,YANG Ai-Guo.Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources[J].Acta Agronomica Sinica,2021(7):1259-1274. |
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| Authors: | WANG Yan-Yan WANG Jun LIU Guo-Xiang ZHONG Qiu ZHANG Hua-Shu LUO Zheng-Zhen CHEN Zhi-Hua DAI Pei-Gang TONG Ying LI Yuan JIANG Xun ZHANG Xing-Wei YANG Ai-Guo |
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| Institution: | (Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,Shandong,China;Deyang Tobacco Company of Sichuan Province,Deyang 618400,Sichuan,China;Sichuan Institute of Tobacco Science,Chengdu 610000,Sichuan,China) |
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| Abstract: | In order to investigate the genetic diversity of Chinese cigar germplasm resources in molecular level and to establish the DNA fingerprint database of cigar varieties,43 pairs of SSR primers with good polymorphic were used to analyze the genetic diversity of 220 cigar germplasms,and 14 pairs of core primers were screened from 43 pairs of SSR primers to construct the fingerprint database of 220 cigar germplasms in the study.Results showed that a total of 243 alleles were amplified by 43 pairs of SSR primers in 220 cigar germplasm materials,with an average of 5.65 per marker,ranging from 2 to 13.The polymorphic information content(PIC)of each locus varied from 0.2078 to 0.9087 with an average of 0.6360.The number of effective alleles(Ne)ranged from 1.3081 to 11.7876,and the average number of effective alleles was 3.9077.The observed heterozygosity(Ho)ranges from 0.0828 to 0.7639 with an average of 0.3191.The expected heterozygosity(He)ranged from 0.2361 to 0.9172 with an average of 0.6809.The average Shannon genetic diversity index(I)was 1.3756,the genetic distance was between 0.0233 and 0.9286,and the average genetic distance was 0.6816.Unweighted pair-group method with arithmetic means(UPGMA)cluster analysis showed that the tested cigar resources can be divided into three groups at the genetic distance of 0.74.All tested materials were divided into two groups by population genetic structure analysis and principal component analysis(PCA).Based on the primer analysis and phenotypic identification,it was determined that the‘LZ’,‘FS’and‘MED’,‘SDDY’and‘MDJ 05-1’,‘Florida 513’and‘CA0701’were the same species with different names.One variety retained one germplasm,leaving 216 different germplasm.14 pairs of SSR primers which could distinguish all the tested materials were screened to construct the fingerprint database of 216 cigar cultivars from 43 pairs of SSR primers as core primers.In conclusion,cigar germplasm resources in China had a high level of genetic diversity.In this study,the constructed SSR fingerprint database and genetic analysis of cigar germplasm resources provided the scientific basis for the screening and identification of high-quality cigar germplasms resources,the mining of important genes,and the broadening the basis of cigar genetic breeding. |
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| Keywords: | cigar germplasm resources SSR marker genetic diversity fingerprint database |
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