| 鳜源维氏气单胞菌的分离鉴定及毒力基因分析 |
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| 引用本文： | 邓汝森,张建州,张浩权,黎家明,罗世诚,颜广智,陈盛楠,黄良宗.鳜源维氏气单胞菌的分离鉴定及毒力基因分析[J].动物医学进展,2022,43(2):35-40. |
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| 作者姓名： | 邓汝森 张建州 张浩权 黎家明 罗世诚 颜广智 陈盛楠 黄良宗 |
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| 作者单位： | 佛山科学技术学院生命科学与工程学院,广东佛山528000 |
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| 基金项目： | 广东君睿生物技术有限公司项目 |
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| 摘 要： | 从发病鳜鱼中分离到1株优势菌(ZQ202010),进行剖检、细菌分离培养、革兰氏染色、生化鉴定、16S rRNA及gyr B基因测序、动物致病性试验、毒力检测和药敏试验.结果显示,分离株ZQ202010为革兰氏阴性小杆菌,具有运动性,该分离菌赖氨酸脱羧酶、氧化酶、VP、吲哚等反应呈阳性;精氨酸水解酶、硫化氢等反应呈阴性...
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| 关 键 词： | 鳜 维氏气单胞菌 鉴定 毒力基因 药敏试验 |
Isolation,Identification and Virulence Gene Analysis of Aeromonas vinieri from Siniperca chuatsi |
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| DENG Ru-sen,ZHANG Jian-zhou,ZHANG Hao-quan,LI Jia-ming,LUO Shi-cheng,YAN Guang-zhi,CHEN Sheng-nan,HUANG Liang-zong.Isolation,Identification and Virulence Gene Analysis of Aeromonas vinieri from Siniperca chuatsi[J].Progress In Veterinary Medicine,2022,43(2):35-40. |
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| Authors: | DENG Ru-sen ZHANG Jian-zhou ZHANG Hao-quan LI Jia-ming LUO Shi-cheng YAN Guang-zhi CHEN Sheng-nan HUANG Liang-zong |
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| Institution: | (College of Life Engineering and Science,Foshan Science and Technology University,Foshan,Guangdong,528000,China) |
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| Abstract: | In order to identify the etiology of outbreak deaths of mandarinfish,a dominant bacterial strain(ZQ202010)was isolated from the infected mandarinfish.This study identified the pathogens through clinical necropsy observation,bacterial isolation and culture,Gram staining observation,biochemical identification,16S rRNA and gyr B gene sequencing analysis,animal regression test,virulence gene detection and drug sensitivity test.The results showed that the strain ZQ202010 was a Gram-negative microbacillus.Biochemical identification results showed that the strain was motile,and lysine decarboxylase,oxidase and VP indole were positive.The reaction of arginine hydrolase and hydrogen sulfide was negative.The 16S rRNA and gyr B gene sequences analysis showed that the isolate and Aeromonas veronii covered into one branch,and the homology was more than 99%.The result of animal regression showed that the LD50 of the isolate to mandarinfish was 4.09×106 CFU,showing high virulence to mandarinfish.The amplification result of virulence gene showed that the isolate carried 7 virulence genes including aer A,Alt,Fla,Exu,Ser,ahy B and Lip,which further indicated that the isolate had high virulence.Drug susceptibility test showed that cefradine,temicocacin,ceftriaxone,florfenicol,doxycycline and other drugs had the most inhibitive effects on the strain.At the same time,it showed resistance to ciprofloxacin,cefotaxime,penicillin,amoxicillin/clavulanic acid,enrofloxacin,cotrimoxazole,rifampicin and ofloxacin.Therefore,a pathogenic Aeromonas veronii strain was isolated in this study,which provides a scientific basis for the prevention and control of Aeromonas vinieri from Siniperca chuatsi. |
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| Keywords: | Siniperca chuatsi Aeromonas veronii identification virulence gene drug susceptibility test |
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