| 小麦株高QTL Qph.nau-5B的效应评价 |
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| 引用本文： | 韩玉洲,张勇,杨阳,顾正中,吴科,谢全,孔忠新,贾海燕,马正强.小麦株高QTL Qph.nau-5B的效应评价[J].作物学报,2021(6):1188-1196. |
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| 作者姓名： | 韩玉洲 张勇 杨阳 顾正中 吴科 谢全 孔忠新 贾海燕 马正强 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室;淮安市农业科学研究院;泰安市农业科学研究院 |
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| 基金项目： | 国家重点研发计划项目(2016YFD0100402);国家自然科学基金项目(31801354,31871620);江苏省“双创计划”资助。 |
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| 摘 要： | 株高直接影响小麦的产量潜力,也是植株抗倒伏性的重要组成部分。目前虽有大量株高相关QTL被鉴定到,但大多QTL的遗传效应仍不清楚。本研究前期利用小麦品种群体,通过关联分析鉴定到一个小麦株高主效QTL Qph.nau-5B。为了评价该QTL的效应,通过分子标记辅助选择分别构建了以南大2419、吉春1016和郑麦9023为供体亲本,中优9507为背景的3种等位变异的近等基因系,背景回复率均高于93%。在7个独立的试验环境中,所有近等基因系的株高较轮回亲本均显著降低,平均降幅为11.1 cm(10.3%)。Qph.nau-5B不同等位变异效应强弱不同,其中来源于吉春1016和郑麦9023的等位变异平均降秆效应相似(12.4 cm),显著大于南大2419的等位变异(8.6 cm),但各等位变异相对降秆效应大小受环境影响。此外,Qph.nau-5B对单株穗数、穗长、千粒重等农艺性状无明显负效应。本研究结果表明Qph.nau-5B具有重要的育种价值,可为小麦的株型分子设计育种提供基因资源。
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| 关 键 词： | 小麦 株高 QTL Qph.nau-5B 近等基因系 分子标记辅助选择 |
Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat |
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| HAN Yu-Zhou,ZHANG Yong,YANG Yang,GU Zheng-Zhong,WU Ke,XIE Quan,KONG Zhong-Xin,JIA Hai-Yan,MA Zheng-Qiang.Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat[J].Acta Agronomica Sinica,2021(6):1188-1196. |
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| Authors: | HAN Yu-Zhou ZHANG Yong YANG Yang GU Zheng-Zhong WU Ke XIE Quan KONG Zhong-Xin JIA Hai-Yan MA Zheng-Qiang |
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| Institution: | (State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;Huai’an Academy of Agricultural Sciences,Huai’an 223001,Jiangsu,China;Tai’an Academy of Agricultural Sciences,Tai’an 271000,Shandong,China) |
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| Abstract: | Plant height affects directly the yield potential of wheat,and constitutes an important component of plant resistance to lodging.Although a large number of QTLs for plant height were detected,most of them have not been evaluated for their genetic effects yet.In the previous study,a major QTL Qph.nau-5B controlling wheat plant height was identified through association mapping in wheat variety collection.To evaluate the dwarfing effect of this QTL,three near-isogenic lines(NILs)with different alleles of Qph.nau-5B were developed using marker-assisted selection with Nanda 2419,Jichun 1016,and Zhengmai 9023 as donor and Zhongyou 9507 as receptor.The recipient genome compositions of these NILs were higher than 93%.Seven independent field trials were conducted and revealed that,compared with the recurrent parent,all NILs indicated a significant decrease in plant height(11.1 cm or 10.3%on average).Three alleles of Qph.nau-5B showed different degrees of dwarfing effects.The alleles coming from Jichun 1016 and Zhengmai 9023 displayed a similar effect on plant height(12.4 cm)in all environments,stronger than that of Nanda 2419(8.6 cm).However,the relative dwarfism effects of different alleles were affected by different environments.Further analysis elucidated that this QTL had little detrimental influence on other agronomical traits such as spike number per plant,spike length and 1000-grain weight.These results suggested the breeding value of Qph.nau-5B that would be utilized for molecular design breeding of plant architecture in wheat. |
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| Keywords: | wheat(Triticum aestivum) plant height QTL Qph nau-5B near-isogenic lines(NILs) marker-assisted selection |
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