| 甜荞花同源异型基因FeMADS1的克隆和序列结构分析 |
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| 引用本文： | 方正武,刘志雄.甜荞花同源异型基因FeMADS1的克隆和序列结构分析[J].西北农业学报,2015,24(3):82-87. |
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| 作者姓名： | 方正武 刘志雄 |
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| 作者单位： | （1.长江大学 农学院，作物遗传育种研究所，湖北荆州 434025；2.长江大学 园艺园林学院，湖北荆州 434025） |
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| 基金项目： | 国家自然科学基金（31101202）；国家公益行业（农业）科研专项（201303008）。 |
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| 摘 要： | 采用同源克隆方法,并结合RACE技术,从甜荞花芽分离得到1个A类MADS-box基因FeMADS1的cDNA全长,GenBank登录号为KM386627,其cDNA全长1 107bp,包括1个编码234个氨基酸、长为705bp的开放阅读框。序列同源比对和分子系统发生分析表明,其蛋白与拟南芥AGL8(FUL)的相似性最高,属A类MADS-box基因亚家族中的euFUL进化系,含MADS、I、K和C末端4个明显的结构域,并且K结构域包含K1、K2和K3共3个保守的富含疏水氨基酸残基的亚结构域,C末端结构域含FUL型基因2个特有的模体:FUL motif和paleoAP1motfi。
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| 关 键 词： | 甜荞 MADS-box基因 FeMADS1 花发育 |
Cloning and Sequence Analysis of FeMADS1 Gene from Fagopyrum esculentum |
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| FANG Zhengwu,and LIU Zhixiong.Cloning and Sequence Analysis of FeMADS1 Gene from Fagopyrum esculentum [J].Acta Agriculturae Boreali-occidentalis Sinica,2015,24(3):82-87. |
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| Authors: | FANG Zhengwu and LIU Zhixiong |
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| Abstract: | The cDNAs of an A -class MADS-box gene, FeMADS1, was cloned by homologous cloning and RACE method based on the sequence database of NCBI. The full length of FeMADS1 cDNA was 1 107 bp, which included an open reading frame (ORF) of 705 bp and coding for a polypeptide of 234 amino acid residues. Conceptual translation revealed that FeMADS1 protein had four domains: MADS, I, K and C-terminal domain . The C terminal region harbored two highly conserved FUL motif and paleoAP1 motif. Three highly conserved regions contained expected hydrophobic amino acids in the K-domain, namely subdomains K1, K2 and K3. |
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| Keywords: | Fagopyrum esculentum MADS-box gene FeMADS1 Floral development |
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