| 抗菌肽Thanatin基因表达载体构建与表达 |
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| 引用本文： | 汪小福,刘仁虎,陈笑芸,高智谋,陈锦清.抗菌肽Thanatin基因表达载体构建与表达[J].安徽农业大学学报,2007,34(4):524-526. |
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| 作者姓名： | 汪小福 刘仁虎 陈笑芸 高智谋 陈锦清 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230036;浙江省农业科学院病毒学与生物技术研究所,杭州,310021;浙江省农业科学院病毒学与生物技术研究所,杭州,310021;安徽农业大学生命科学学院,合肥,230036 |
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| 摘 要： | 根据大肠杆菌的密码子偏好性,人工设计并合成了3条寡核苷酸片段,相邻片断有17个碱基的重叠区,通过PCR扩增得到抗菌肽Thanatin基因,将此基因克隆到原核表达载体pGEX-4T-1中,然后将重组质粒pGEX- Thanatin转化E.coli BL 21,经IPTG诱导进行融合表达.通过SDS - PAGE分析,融合蛋白GST -Thanatin 表达成功.
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| 关 键 词： | Thanatin 融合蛋白 表达 抗菌肽 |
| 文章编号： | 1672-352X（2007）044）524-03 |
| 修稿时间： | 2007-01-05 |
Vectors construction and expression of antimicrobial peptide Thanatin |
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| WANG Xiao-fu,LIU Ren-hu,CHEN Xiao-yun,GAO Zhi-mou,CHEN Jin-qing.Vectors construction and expression of antimicrobial peptide Thanatin[J].Journal of Anhui Agricultural University,2007,34(4):524-526. |
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| Authors: | WANG Xiao-fu LIU Ren-hu CHEN Xiao-yun GAO Zhi-mou CHEN Jin-qing |
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| Institution: | 1. School of Life Science, Anhui Agricultural University, Hefei 230036 ; 2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 |
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| Abstract: | Three gene fragments were designed and synthesized based on the preference of the codon of E.coli,and the gene of Thanatin was obtained by PCR amplification and cloned into prokaryotic expression vector pGEX-4T-1.Then the recombinant expression plasmid pGEX-Thanatin was transformed into E.coli BL21 and the fusion protein of Thanatin was produced by IPTG induction.The analysis of SDS-PAGE showed that the fusion pGEX-Thanatin protein was expressed,which might lay a foundation on research of antimicrobial activities and its mechanism. |
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| Keywords: | Thanatin fusion protein expression antimicrobial peptide |
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