| 渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究 |
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| 引用本文： | 孙朝霞,侯思宇,王玉国.渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究[J].安徽农业科学,2008,36(26). |
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| 作者姓名： | 孙朝霞 侯思宇 王玉国 |
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| 作者单位： | 山西农业大学农学院生物技术系,山西太谷,030801;中国农业科学院生物技术研究所,北京,100081 |
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| 摘 要： | 目的]研究渗透调节物质及有机附加物对小麦体细胞胚发生的影响。方法]通过在小麦体细胞胚发生体系中添加不同浓度的渗透调节物质甘露醇或山梨醇及谷氨酰胺(Gln)、水解酪蛋白(CH)和水解乳蛋白(LH)等天然有机物,探讨影响小麦体细胞胚发生的最佳附加物配比组合。结果]添加甘露醇或山梨醇≤40 g/L时,有利于促进体细胞胚的发生;单独添加300~500 mg/L Gln、CH或LH对体细胞胚的诱导无明显差别,但同时添加500 mg/LGln和300 mg/LCH可明显提高体细胞胚的发生。结论]添加不同浓度的渗透调节物质和有机附加物可在一定程度上促进体细胞胚的发生,为完善小麦体细胞胚发生体系奠定基础。
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| 关 键 词： | 小麦 体细胞胚发生 有机附加物 渗透调节物 |
Study on the Effect of Osmosis-regulating Substances and organic Appendices on Somatic Embryogenesis in Wheat |
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| SUN Zhao-xia et al.Study on the Effect of Osmosis-regulating Substances and organic Appendices on Somatic Embryogenesis in Wheat[J].Journal of Anhui Agricultural Sciences,2008,36(26). |
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| Authors: | SUN Zhao-xia |
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| Abstract: | Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat.Method] The sllitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances such as mannitol,sorbitol and organic appendices such as Gln,CH and LH into the somatic embryogenesis in wheat.Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis;there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium,while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH.Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices,which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat. |
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| Keywords: | Wheat Somatic embryogenesis Organic appendix Osmosis-regulating substance |
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