| 两种微卫星多样PCR系统应用于胡杨高效生产基因型 |
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| 作者姓名： | Eusemann Pascal Fehrenz Steffen Schnittler Martin |
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| 作者单位： | Department of Botany and Landscape Ecology, EMAU Greifswald, Grimmer Str. 88, 17487 Greifswald, Germany |
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| 基金项目： | supported by the Deutsche For-schungsgemeinschaft DFG (grant number SCHN 1080/1-1) |
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| 摘 要： | 对已在橡树岭国家实验室建立起来的美洲山杨(Populus tremuloides Michx.)和毛果杨(Populus trichocarpa Torr.＆ Gray)18个微卫星引物进行筛选,用于胡杨相关基因的扩增.发现有13个基因位点可以表达基因多态性,范围是2～17个等位基因.选择了8个最易变的基因位点,来构建和优化2种多样PCR法.用来自3个种群的436株树木表征选择的位点,确定他们在亲缘关系分析、基因型研究的适用性.通过克隆基因型鉴定树木性别的交叉检验,我们估计并入基因型最大误差率小于O.045.
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| 关 键 词： | 克隆标识 胡杨 基因指纹 亲缘关系分析 种群结构 SSR引物 |
Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica |
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| Eusemann Pascal,Fehrenz Steffen,Schnittler Martin.Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica[J].Journal of Forestry Research,2009,20(3). |
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| Authors: | Eusemann Pascal Fehrenz Steffen Schnittler Martin |
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| Institution: | Eusemann Pascal*,Fehrenz Steffen,Schnittler Martin Department of Botany , L,scape Ecology,EMAU Greifswald,Grimmer Str. 88,17487 Greifswald,Germany |
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| Abstract: | Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Torr. & Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were selected to set up and optimize two multiplex polymerase chain reaction (PCR) assays. Three populations containing altogether 436 trees were used to characteri... |
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| Keywords: | clone identification Euphrates poplar genetic fingerprint parentage analysis population structure SSR primers |
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