| 猕猴桃根中三萜类化合物的提取工艺研究 |
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| 引用本文： | 田娜,陈颖韬,吴文杰,张盛,刘硕谦.猕猴桃根中三萜类化合物的提取工艺研究[J].中国农学通报,2013,29(25):184-189. |
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| 作者姓名： | 田娜 陈颖韬 吴文杰 张盛 刘硕谦 |
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| 作者单位： | [1]湖南农业大学植物资源工程系,长沙410128 [2]国家植物功能成分利用工程技术研究中心,长沙410128 |
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| 基金项目： | 国家科技支撑重点项目“武陵山区特色资源高效综合利用关键技术研究与示范”（2011BAD10B01） |
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| 摘 要： | 对猕猴桃根中的三萜类化合物的提取工艺进行研究,获得最佳的提取工艺条件,采用单因素实验考察提取温度、乙醇体积分数、提取时间、料液比与提取次数对三萜类化合物得率的影响,并以正交实验进一步优化提取工艺条件。猕猴桃根三萜类物质的最优提取工艺条件为提取温度80℃,乙醇体积分数65%,提取时间1.5 h,料液比1：20（g/mL）,提取次数1次,在此工艺条件下,猕猴桃根三萜类物质的平均得率为6.7517%。该工艺应用于猕猴桃根中三萜类物质的提取简单可行,有较好的参考价值。
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| 关 键 词： | 猕猴桃根 三萜 提取工艺 正交实验 |
| 收稿时间： | 1/9/2013 12:00:00 AM |
| 修稿时间： | 2013/3/22 0:00:00 |
Optimization of Extraction Process for Triterpenoids from the Roots of Radix Actinidiae Chinensis |
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| Tian Na,Chen Yingtao,Wu Wenjie,Zhang Sheng,Liu Shuoqian.Optimization of Extraction Process for Triterpenoids from the Roots of Radix Actinidiae Chinensis[J].Chinese Agricultural Science Bulletin,2013,29(25):184-189. |
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| Authors: | Tian Na Chen Yingtao Wu Wenjie Zhang Sheng Liu Shuoqian |
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| Institution: | 1 Department of Botanic Engineering, Hunan Agricultural University, Changsha 410128; 2National Research Center of Engineering Technology for Utilization of Foundational Ingredients from Botanicals, Changsha 410128) |
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| Abstract: | The study was aimed to optimize the extraction of the triterpenoids from the roots of Radix Aetinidiae chinensis and obtain the optimal extraction process. The effect of extraction temperature, ethanol concentration （volume percent）, extraction time, material-to-liquid ratio and the number of extraction times on the yield of triterpenoids is investigated through single factor tests and orthogonal tests. The optimal extraction conditions were determined as follows： extraction temperature of 80℃, ethanol concentration of 65%, extraction time of 1.5 h, material-to-liquid ratio of 1：20 （g/mL） and the number of extraction times was one time. Under the optimized extraction conditions, 6.7517% of the extraction yield of triterpenoids from Radix Actinidiae chinensis was achieved. This optimized extraction process was simple, feasible and had a very good reference value. |
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| Keywords: | Radix Actinidiae chinensis triterpenoids extraction orthogonal test |
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