| 产吲哚乙酸（IAA）促生菌的分离鉴定及对烟草种子萌发和幼苗生长发育的影响 |
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| 引用本文： | 陈越,李虎林,朱诗苗,闫寒,郎彬,姬文秀.产吲哚乙酸（IAA）促生菌的分离鉴定及对烟草种子萌发和幼苗生长发育的影响[J].作物杂志,2020,36(2):176-7633. |
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| 作者姓名： | 陈越 李虎林 朱诗苗 闫寒 郎彬 姬文秀 |
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| 作者单位： | 延边大学农学院,133002,吉林延吉 |
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| 基金项目： | 吉林省烟草专卖局科技创新计划(JLYC-KJ-2014-7) |
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| 摘 要： | 为获得用于烟草的优良微生物肥料菌种资源,探讨其对烟草种子萌发、幼苗生长的影响,从延边地区烟草根际土壤中分离筛选具有产吲哚乙酸（IAA）能力的菌株并进行分子鉴定,研究该单一菌及混合菌剂对烟草种子萌发和幼苗生长发育的影响。结果表明,鞘氨醇杆菌属（Sphingobacterium sp.）m-53与咸海鲜球菌属（Jeotgalicoccus sp.）m-60产IAA效果较好,m-53、m-60及二者混合菌剂的最佳培养时间分别为24、48和48h,其对应的IAA产量分别为98、41和141mg/L。与对照相比,单一菌和混合菌剂均能促进烟草种子萌发及幼苗生长,尤以混合菌剂的效果最佳。m-53和m-60可单独施用或混合施用均作为烟草微生物肥料的备选资源。
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| 关 键 词： | 烟草 根际促生菌 吲哚乙酸 促生效应 |
| 收稿时间： | 2019-09-25 |
Isolation and Identification of IAA-Producing Rhizobacteria and Its Effects on Seed Germination and Seedling Growth of Tobacco |
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| Chen Yue,Li Hulin,Zhu Shimiao,Yan Han,Lang Bin,Ji Wenxiu.Isolation and Identification of IAA-Producing Rhizobacteria and Its Effects on Seed Germination and Seedling Growth of Tobacco[J].Crops,2020,36(2):176-7633. |
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| Authors: | Chen Yue Li Hulin Zhu Shimiao Yan Han Lang Bin Ji Wenxiu |
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| Institution: | College of Agriculture, Yanbian University, Yanji 133002, Jilin, China |
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| Abstract: | In order to obtain the high-quality bacteria resources and to explore their effects on the seed germination and seedling growth of tobacco, we isolated and screened IAA-producing strains from rhizosphere soil of tobacco in Yanbian area and carried out 16S rRNA sequencing identification, as well as investigated the effects of the single and mixed bacteria on the germination and seedling growth of tobacco. The results showed that both Sphingobacterium sp. m-53 and Jeotgalicoccus sp. m-60 produced more IAA than other strains, and the optimal culture time of m-53, m-60 and their mixture for growth were 24, 48 and 48h, and their corresponding IAA yields were 98, 41 and 141mg/L, respectively. Compared with the control, both single and mixed bacteria could promote seed germination and seedling growth of tobacco, especially the mixed bacteria had the best promotion effect. Sphingobacterium sp. m-53 and Jeotgalicoccus sp. m-60 can be applied separately or in combination as an alternative resource for microbial fertilizers of tobacco. |
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| Keywords: | Tobacco Plant growth-promoting rhizobacteria Indole acetic acid Promoting effect |
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