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海南岛西瓜病毒病种类鉴定及发生分布
引用本文:车海彦,曹学仁,贺延恒,罗大全.海南岛西瓜病毒病种类鉴定及发生分布[J].植物病理学报,2020,50(5):632-636.
作者姓名:车海彦  曹学仁  贺延恒  罗大全
作者单位:中国热带农业科学院环境与植物保护研究所, 海口 571101
基金项目:2018年海南省重大科技计划项目(zdkj201813);中国热带农业科学院基本业务费专项项目(19CXTD-33)
摘    要:正病毒病是西瓜生产上的重要病害,目前侵染我国西瓜的病毒主要有西瓜花叶病毒(watermelon mosaic virus,WMV)、黄瓜花叶病毒(cucumber mosaic virus,CMV)、黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV) 、甜瓜黄斑病毒(melon yellow spot virus,MYSV)、小西葫芦黄花叶病毒

收稿时间:2019-10-25

Distribution and identification of watermelon viruses in Hainan island
CHE Hai-yan,CAO Xue-ren,HE Yan-heng,LUO Da-quan.Distribution and identification of watermelon viruses in Hainan island[J].Acta Phytopathologica Sinica,2020,50(5):632-636.
Authors:CHE Hai-yan  CAO Xue-ren  HE Yan-heng  LUO Da-quan
Institution:Environment and Plant Protection Institute, CATAS, Haikou 571101, China
Abstract:From October 2017 to April 2019, a total of 290 suspected virus-infected watermelon leaf samples were collected in 7 urban areas (Wenchang, Wanning, Lingshui, Sanya, Ledong, Dongfang, Changjiang) in Hainan island. Nine viruses were detected including MYSV, WSMoV, CCYV, CMV, TMV, CGMMV, ZYMV, WMV and PRSV by small RNA deep sequencing and RT-PCR. The detection rates of MYSV, CCYV, CGMMV, ZYMV, CMV, WSMoV, PRSV, TMV and WMV were 66.90%, 25.86%, 22.07%, 17.59%, 10.00%, 7.59%, 5.52%, 2.76% and 1.03% respectively. The co-infection rate of virus was 56.56% and 14 types of mixed infections were detected. The results of this study clarify the identification and distribution of waterme-lon-infecting viruses in Hainan.
Keywords:Hainan  watermelon virus diseases  small RNA deep sequencing  virus identification  
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