| 黑龙江省稻瘟病菌无毒基因的检测 |
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| 引用本文： | 孟峰,张亚玲,靳学慧.黑龙江省稻瘟病菌无毒基因的检测[J].作物杂志,2020,36(3):197-74. |
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| 作者姓名： | 孟峰 张亚玲 靳学慧 |
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| 作者单位： | 黑龙江八一农垦大学/黑龙江省作物-有害生物互作生物学及生态防控重点实验室/黑龙江省植物抗性研究中心,163319,黑龙江大庆 |
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| 基金项目： | 黑龙江八一农垦大学学成、引进人才科研启动计划(XDB-2016-05);黑龙江省自然科学基金(QC2011C046);黑龙江省农垦总局科技攻关项目(HNK125A-08-06);黑龙江省农垦总局科技攻关项目(HNK135-02-02);黑龙江省教育厅项目(12521376) |
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| 摘 要： | 为了检测黑龙江省稻瘟病菌无毒基因ACE1、AVR-Pi9、AVR-Pia、AVR-Pii和AVR1-CO39的存在情况,采用5对无毒基因引物,对2017年黑龙江省208个稻瘟病菌单孢菌株DNA进行PCR扩增检测。结果表明,ACE1出现频率最高（87.98％）;AVR1-CO39出现频率最低（0）;无毒基因AVR-Pi9的出现频率为79.81％;AVR-Pia的出现频率为16.35％;AVR-Pii的出现频率为0.48％。黑龙江省稻瘟病菌无毒基因群体组成结构错综复杂,且具有明显的地域特点。
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| 关 键 词： | 黑龙江省 稻瘟病菌 无毒基因 检测 |
| 收稿时间： | 2019-10-12 |
Detection of Avirulence Genes of Magnaporthe oryzea in Heilongjiang Province |
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| Meng Feng,Zhang Yaling,Jin Xuehui.Detection of Avirulence Genes of Magnaporthe oryzea in Heilongjiang Province[J].Crops,2020,36(3):197-74. |
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| Authors: | Meng Feng Zhang Yaling Jin Xuehui |
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| Institution: | Heilongjiang Bayi Agricultural University/Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control/Heilongjiang Plant Resistance Research Center, Daqing 163319, Heilongjiang, China |
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| Abstract: | In order to detect the presence of the avirulence genes ACE1, AVR-Pi9, AVR-Pia, AVR-Pii and AVR1-CO39 of Magnaporthe oryzae in Heilongjiang Province, the DNA of 208 strains of Magnaporthe oryzae in Heilongjiang Province was detected by PCR using 5 pairs avirulence gene primers. The results showed that the highest frequency of ACE1 was 87.98%; the frequency of AVR1-CO39 was 0; the frequency of avirulence gene AVR-Pi9 was 79.81%; the frequency of amplification of AVR-Pia was 16.35% and the amplification frequency of AVR-Pii was 0.48%. The composition of the avirulence gene population of Magnaporthe oryzae in Heilongjiang Province is complex and has obvious regional characteristics. |
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| Keywords: | Heilongjiang Province Magnaporthe oryzae Avirulence gene Detection |
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