| 高粱硬脂酰-ACP脱氢酶基因（SbSAD）家族鉴定及不同发育阶段表达分析 |
| |
| 引用本文： | 赵训超,徐晶宇,盖胜男,魏玉磊,许晓萱,丁冬,刘梦,张今杰,邵文静.高粱硬脂酰-ACP脱氢酶基因（SbSAD）家族鉴定及不同发育阶段表达分析[J].作物杂志,2020,36(2):20-104. |
| |
| 作者姓名： | 赵训超 徐晶宇 盖胜男 魏玉磊 许晓萱 丁冬 刘梦 张今杰 邵文静 |
| |
| 作者单位： | 黑龙江八一农垦大学农学院/黑龙江省现代农业栽培技术与作物种质改良重点实验室,163319,黑龙江大庆 |
| |
| 基金项目： | 黑龙江八一农垦大学优势特色学科建设项目专项资金(2042070002) |
| |
| 摘 要： | 硬脂酰-ACP脱氢酶是形成不饱和脂肪酸的关键酶。利用拟南芥蛋白序列作为比对序列在NCBI和Phytozome数据库进行Blastp同源比对,共鉴定11个SbSADs基因。对SbSAD基因家族进行蛋白特性、进化关系、不同发育阶段表达、基因结构、保守基序、染色体定位、基因二级和三级结构、启动子区的顺式元件及种子发育阶段表达分析,结果显示,SbSADs基因编码区为1 134~1 290bp,其编码蛋白的氨基酸数为377~449,分子量最大为48.0kDa,最小为39.8kDa,等电点为5.26~8.76;根据系统发育树,将SbSAD基因家族分为3个亚族,其中亚族I在不同组织发育阶段表达量较高;11个SbSADs基因不均等地定位在高粱1、2、3、4、6、7和10号染色体上;SbSADs蛋白二级结构均以α-螺旋和无规则卷曲为主,三级结构预测显示,除SbSAD8外,其余SbSADs的蛋白三级结构高度相似;11个SbSADs基因的启动子区与低温胁迫相关的元件数量高度富集。SbSAD5基因在种子发育5和10d均有较高的表达量。
|
| 关 键 词： | 高粱 硬脂酰-ACP脱氢酶 生物信息学 基因家族 |
| 收稿时间： | 2019-08-19 |
Identification of Stearyl-ACP Desaturase Gene (SbSAD) Family and Their Expression Analysis at Different Developmental Stages in Sorghum |
| |
| Zhao Xunchao,Xu Jingyu,Gai Shengnan,Wei Yulei,Xu Xiaoxuan,Ding Dong,Liu Meng,Zhang Jinjie,Shao Wenjing.Identification of Stearyl-ACP Desaturase Gene (SbSAD) Family and Their Expression Analysis at Different Developmental Stages in Sorghum[J].Crops,2020,36(2):20-104. |
| |
| Authors: | Zhao Xunchao Xu Jingyu Gai Shengnan Wei Yulei Xu Xiaoxuan Ding Dong Liu Meng Zhang Jinjie Shao Wenjing |
| |
| Institution: | College of Agronomy, Heilongjiang Bayi Agricultural University/Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, Daqing 163319, Heilongjiang, China |
| |
| Abstract: | Stearyl-ACP desaturase is a key enzyme to form unsaturated fatty acids. A total of 11 full-length SbSADs genes were identified by Blastp from the NCBI and Phytozome database using the SbSADs genes as queries. The protein properties, phylogenetics, tissue-specific expression patterns of SbSADs, gene structure, conserved motifs, chromosome mapping, secondary and tertiary structures, cis-elements in promoter regions and their expression level at various seed development stages were analyzed. The results showed that open reading frame of SbSADs genes was among 1 134-1 290bp, the number of amino acids of their encoded proteins ranged from 377 to 449, the maximum molecular weight was 48.0kDa and the minimum was 39.8kDa, and the isoelectric point was 5.26-8.76. According to phylogenetic analysis, SbSADs were divided into 2 sub-groups, and the expression level of sub-group I was higher in different tissues. SbSADs genes were unequally mapped on 1, 2, 3, 4, 6, 7 and 10 chromosomes of sorghum. The prediction of secondary structure showed that SbSADs proteins was mainly composed of α-helix and irregular coil, while the tertiary structures prediction showed that except SbSAD8, the rest of SbSADs proteins were highly similar. In the SbSADs gene family, there were a number of cis-elements related to low temperature stress. The expression of SbSAD5 gene was higher at 5 and 10d of seed development. |
| |
| Keywords: | Sorghum Stearyl-ACP desaturase Bioinformatics Gene family |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《作物杂志》浏览原始摘要信息 |
| 点击此处可从《作物杂志》下载免费的PDF全文 |
|
