| 甘薯病毒2中国分离物全基因组序列克隆及遗传进化分析 |
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| 引用本文： | 秦艳红,乔奇,王爽,张德胜,王永江,田雨婷,张振臣.甘薯病毒2中国分离物全基因组序列克隆及遗传进化分析[J].植物病理学报,2020,50(4):505-508. |
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| 作者姓名： | 秦艳红 乔奇 王爽 张德胜 王永江 田雨婷 张振臣 |
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| 作者单位： | 河南省农业科学院植物保护研究所,郑州 450002;河南省农作物病虫害防治重点实验室,郑州 450002;农业部华北南部作物有害生物综合治理重点实验室,郑州 450002 |
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| 基金项目： | 国家甘薯产业技术体系建设项目(CARS-10-B13);河南省农业科学院自主创新基金项目(2019ZC37);河南省科技攻关项目(182102110274) |
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| 摘 要： | 正甘薯病毒2(Sweet potato virus 2,SPV2)是马铃薯Y病毒科(Potyviridae)马铃薯Y病毒属(Potyvirus)成员。SPV2也称为甘薯脉花叶病毒(ipomoea vein mosaic virus,IVMV)和甘薯Y病毒(sweet potato virus Y,SPVY)~1],是甘薯上常见的病毒之一。SPV2病毒粒体为线条状,长度为850 nm,在细胞质中形成风轮状或卷轴状内含体~2]。SPV2可由桃
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| 收稿时间： | 2019-07-06 |
Complete nucleotide sequence and phylogenetic analysis of the sweet potato virus 2 isolated from China |
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| QIN Yan-hong,QIAO Qi,WANG Shuang,ZHANG De-sheng,WANG Yong-jiang,TIAN Yu-ting,ZHANG Zhen-chen.Complete nucleotide sequence and phylogenetic analysis of the sweet potato virus 2 isolated from China[J].Acta Phytopathologica Sinica,2020,50(4):505-508. |
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| Authors: | QIN Yan-hong QIAO Qi WANG Shuang ZHANG De-sheng WANG Yong-jiang TIAN Yu-ting ZHANG Zhen-chen |
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| Institution: | Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; Henan Key Laboratory of Crop Pest Control, Zhengzhou 450002, China; IPM Key Laboratory in Southern part of North China for Ministry of Agriculture, Zhengzhou 450002, China |
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| Abstract: | The complete nucleotide sequence of a Chinese isolate of sweet potato virus 2 (SPV2-Ch1) was determined by using RT-PCR and rapid amplification of cDNA ends methods. Sequence comparisons of complete genomic sequence, polyprotein, and individual protein sequences were performed using DNAMAN. Phylogenetic tree of SPV2-Ch1 isolate with other SPV2 isolates were generated using MEGA7.0 software. Sequence analysis revealed that the complete genome of SPV2-Ch1 was 10 747 nucleotides (nt) including a poly(A) tail at the 3'-end. The 5'-untranslated region (5'-UTR) and 3'-UTR is 107 nt and 239 nt in length, respectively. The genome of SPV2-Ch1 contained an open reading frame of 10 401 nt encoding for a putative polyprotein of 3 466 amino acids. Pairwise comparisons showed that SPV2-Ch1 and other SPV2 isolates available in GenBank database shared 80.3%-98.7% identity at the complete genome nucleotide sequence level. SPV2-Ch1 has the highest nt identity with the isolate SC6 (98.7%) and lowest nt similarity with isolate SSBles-74_ZA (80.3%). Phylogene-tic tree analysis based on polyprotein gene indicated that isolates SPV2-Ch1, AM-MB2, AuScan, CW142, GJ118, GWB-2, HN77, LSU2, SC6, SCN20 and TM37 formed one group. This is the first report of the complete genomic sequence of SPV2 isolated from China. |
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| Keywords: | sweet potato virus 2 complete nucleotide sequence genomic characterization phylogenetic analysis |
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