| 植物微根系原位观测方法研究进展 |
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| 引用本文： | 肖爽,刘连涛,张永江,孙红春,白志英,张科,田士军,董合忠,李存东.植物微根系原位观测方法研究进展[J].植物营养与肥料学报,2020,26(2):370-385. |
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| 作者姓名： | 肖爽 刘连涛 张永江 孙红春 白志英 张科 田士军 董合忠 李存东 |
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| 作者单位： | 1.河北农业大学/河北省作物生长调控重点实验室，河北保定 071000 |
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| 基金项目： | “十三五”国家重点研发计划项目（2018YFD0100306）；国家自然科学基金项目（31871569、31571610）；河北省产业技术体系（HBCT2018040201）。 |
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| 摘 要： | 植物根系是固定植株、获取水分和营养物质的主要器官。作为连接植物体与土壤环境的枢纽，根系的发育状况直接关系着地上部分的形态建成。由细根及其根毛组成的微根系是根系功能的关键组织。由于土壤中根系的非直观性和根系自身的复杂立体构型，常规研究方法很难准确测定根系的变化动态。微根系原位观测技术、方法的出现为原位根系研究提供了可能的手段。近年来，可用于观测微根系二维形态与立体构型的多种类型的原位生长系统、装置被研制出来，一些根系图像分析软件也不断开发和改进，大大提升了原位根系研究的准确性。本文介绍了几种用于原位微根系观测的装置和图像分析软件与计算方法，并比较了不同方法与软件之间的优劣，指出原位观测研究技术的应用和发展趋势，以期对后续植物微根系研究提供参考。
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| 关 键 词： | 微根系 原位观测 表型平台 分析方法 图像分析 研究进展 |
| 收稿时间： | 2019-05-10 |
Review on new methods of in situ observation of plant micro-roots and interpretation of root images |
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| XIAO Shuang,LIU Lian-tao,ZHANG Yong-jiang,SUN Hong-chun,BAI Zhi-ying,ZHANG Ke,TIAN Shi-jun,DONG He-zhong,LI Cun-dong.Review on new methods of in situ observation of plant micro-roots and interpretation of root images[J].Plant Nutrition and Fertilizer Science,2020,26(2):370-385. |
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| Authors: | XIAO Shuang LIU Lian-tao ZHANG Yong-jiang SUN Hong-chun BAI Zhi-ying ZHANG Ke TIAN Shi-jun DONG He-zhong LI Cun-dong |
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| Institution: | 1.Hebei Agricultural University/Key Laboratory of Crop Growth Regulation, Baoding, Hebei 071000, China |
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| Abstract: | Root system plays key roles in fixing plants, obtaining water and nutrients for plants. As a hub connecting plant shoots and soil environment, the development of root system determines the formation of the aboveground part. Micro-root system is the main execution site of root function, consists of fine roots and root hairs. Due to the non-intuitive nature of the roots and complex stereo configuration of the root system in the soil, conventional research methods are ineffective for observing the real morphology of root systems. In-situ and non-destructive methods have been attempted and attracted more and more attention in present years. The main types of in-situ and non-destructive devices that can be used to observe the 2D morphology and stereo configuration of micro-roots, and some root image analysis software were introduced and evaluated for efficient use of them in root research. |
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