| 分子标记辅助选择Pi9基因改良湘晚籼11号的稻瘟病抗性 |
| |
| 引用本文： | 李枳蒙,张婷,李典,黄俊,赖怡帆,吴婷婷,李博文,刘雄伦.分子标记辅助选择Pi9基因改良湘晚籼11号的稻瘟病抗性[J].作物研究,2020(4):359-362,387. |
| |
| 作者姓名： | 李枳蒙 张婷 李典 黄俊 赖怡帆 吴婷婷 李博文 刘雄伦 |
| |
| 作者单位： | 湖南农业大学农学院;作物基因工程湖南省重点实验室;水稻油菜抗病育种湖南省重点实验室 |
| |
| 基金项目： | 国家转基因育种重大专项(2016ZX08001-002)。 |
| |
| 摘 要： | 以携带广谱持久抗稻瘟病基因Pi9的籼稻品系75-1-127为供体亲本,以优质常规晚稻湘晚籼11号为受体亲本及轮回亲本,利用Pi9基因内特异标记,在连续回交自交群体中开展分子标记辅助选择,定向改良湘晚籼11号的稻瘟病抗性。24份稻瘟菌菌株室内接种及田间病圃抗性鉴定结果表明,75-1-127和湘晚籼11号的抗菌谱差异显著,抗性频率分别为91.7%和29.2%,75-1-127高抗苗瘟和穗瘟,而湘晚籼11号高感苗瘟和穗瘟;鉴定出1个稳定高效的显性DNA标记Ins2-1,该标记引物从75-1-127基因组中扩增出1条532 bp的清晰条带,而在湘晚籼11号基因组中无扩增产物。利用Ins2-1开展标记辅助选择育种实践,从湘晚籼11号×75-1-127的BC6F3群体中鉴定出9个抗病改良系,为进一步培育优异抗病水稻新品种打下了基础。
|
| 关 键 词： | 水稻 稻瘟病 抗性改良 分子标记辅助选择 Pi9 |
Improving Blast Resistance of Xiangwanxian 11 by Molecular Marker-assisted Selection of the Pi9 Gene |
| |
| LI Zhimeng,ZHANG Ting,LI Dian,HUANG Jun,LAI Yifan,WU Tingting,LI Bowen,LIU Xionglun.Improving Blast Resistance of Xiangwanxian 11 by Molecular Marker-assisted Selection of the Pi9 Gene[J].Crop Research,2020(4):359-362,387. |
| |
| Authors: | LI Zhimeng ZHANG Ting LI Dian HUANG Jun LAI Yifan WU Tingting LI Bowen LIU Xionglun |
| |
| Institution: | (College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128,China;Rice and Rapeseed Disease Resistance Breeding Key Laboratory of Hunan Province,Changsha,Hunan 410128,China) |
| |
| Abstract: | Pi9 is a cloned broad-spectrum and durable blast resistance gene.To improve blast resistance of the indica rice variety Xiangwanxian 11,Pi9-specific DNA marker was employed for molecular marker-assisted selection breeding in the consecutive backcrossing and self-pollination populations of Xiangwanxian 11×75-1-127. Resistance responses to 24 tested M.oryzae strains were quite different between the Pi9 donor 75-1-127 and the receptor Xiangwanxian 11,presenting 91.7% and 29.2% resistance ratio respectively in the greenhouse inoculation assay,and 75-1-127 showed high-level seedling and panicle blast resistance but Xiangwanxian 11 was highly susceptible in field nursery. An efficient PCR-based dominant marker Ins2-1 was identified,which can amplify a stable 532 bp band from 75-1-127 genome but nothing from that of Xiangwanxian 11. Nine improved blast resistant rice lines were confirmed from the BC6F3 population of Xiangwanxian 11×75-1-127 using the marker Ins2-1,which paved a way for further breeding elite rice varieties. |
| |
| Keywords: | rice blast resistance improvement molecular marker-assisted selection Pi9 |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
