| 基于形态与分子数据鉴定海榄雌瘤斑螟 |
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| 引用本文： | 王林聪,王孟琪,杨琼,昝启杰,郑童,林伟,徐淼锋.基于形态与分子数据鉴定海榄雌瘤斑螟[J].植物检疫,2020(1):14-17. |
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| 作者姓名： | 王林聪 王孟琪 杨琼 昝启杰 郑童 林伟 徐淼锋 |
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| 作者单位： | 广东内伶仃福田国家级自然保护区管理局;拱北海关 |
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| 基金项目： | 深圳市科技计划项目(JSGG20170413103811649);原国家质检总局科技计划项目(2016IK300)。 |
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| 摘 要： | 海榄雌瘤斑螟目前是红树林植物白骨壤的重要害虫,其种类在国内存在争议。本研究对福田红树林自然保护区内采集到的螟蛾标本进行了形态学鉴定,同时应用DNA条形码技术进行了进一步确认。本文将海榄雌瘤斑螟样本的线粒体COI序列与数据库中已有序列比对,结果显示海榄雌瘤斑螟COI序列与GenBank数据库中3条P. synataractis和BOLD系统中的32条P. syntaractis序列同源性达98%以上;构建的系统发育树显示福田红树林自然保护区内采集的标本与P. syntaractis聚为一支。综合形态和分子数据确定海榄雌瘤斑螟为P. syntaractis (Turner,1904)。
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| 关 键 词： | 海榄雌瘤斑螟 福田红树林 白骨壤 DNA条形码 鉴定 |
Morphological and molecular identification of Ptyomaxia syntaractis |
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| Wang Lincong,Wang Mengqi,Yang Qiong,Zan Qijie,Zheng Tong,Lin Wei,Xu Miaofeng.Morphological and molecular identification of Ptyomaxia syntaractis[J].Plant Quarantine,2020(1):14-17. |
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| Authors: | Wang Lincong Wang Mengqi Yang Qiong Zan Qijie Zheng Tong Lin Wei Xu Miaofeng |
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| Institution: | (Guangdong Neilingding-Futian National Nature Reserve Administrative Bureau,Shenzhen 518040,China;Gongbei Customs District) |
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| Abstract: | Ptyomaxia sp. is an important insect pest of mangrove plant Avicennia marina(Forsk.) Vierh,while the classification of this insect is still controversial in China. In this study,the samples from Futian Mangrove Nature Reserve were identified by morphological characters and DNA barcoding. The mitochondrial COI sequence of this insect was amplified and compared in the GenBank and the BOLD System. The results showed that the sequences were more than 98% similar with 3 P. syntaractis records in the GenBank database and 32 records in BOLD System. The phylogenetic tree showed that all the samples from Futian Mangrove Nature Reserve were clustered into one branch with the P. syntaractis. Based on morphological and molecular data,this species of Ptyomaxia sp. was identified as Ptyomaxia syntaractis(Turner,1904). |
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| Keywords: | Ptyomaxia syntaractis Futian mangrove Avicennia marina DNA barcoding identification |
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