| 水稻可消化蛋白质含量测定方法研究及资源筛选 |
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| 引用本文： | 陈庭木,孙志广,邢运高,方兆伟,王宝祥,刘艳,徐大勇.水稻可消化蛋白质含量测定方法研究及资源筛选[J].作物杂志,2020,36(1):61-23. |
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| 作者姓名： | 陈庭木 孙志广 邢运高 方兆伟 王宝祥 刘艳 徐大勇 |
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| 作者单位： | 江苏省连云港市农业科学院/江苏省现代作物生产协同创新中心,222006,江苏连云港 |
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| 基金项目： | 现代农业技术体系建设专项资金;江苏省科技计划重点项目:清香软粳的水稻基因发掘、育种材料创制及其方法(BE2017323);国家七大育种专项:长江中下游优质高产高效粳稻新品种培育(2017YFD0100400) |
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| 摘 要： | 水稻是重要口粮作物,是重要的蛋白质来源,但水稻种子蛋白质中包括不可消化的醇溶蛋白与可消化的谷蛋白、清蛋白与球蛋白。通过蛋白质提取方法研究,探讨一种可行的水稻可消化蛋白质含量测定方法,并对从国际水稻所引进的804份品种资源进行测定。结果表明,60%乙醇水溶液中加入0.4%质量体积分数的NaOH作为提取溶剂,采用300W超声波重复提取3次可以获得可消化蛋白质含量的准确测定结果,测得804份品种资源的平均可消化蛋白质含量为7.80%。本研究为水稻的可消化蛋白质含量测定提供了快速有效的技术手段,并筛选出了一批可消化蛋白质含量高和可消化蛋白质含量低的水稻资源。
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| 关 键 词： | 水稻蛋白质 可消化蛋白 考马斯亮蓝G250 测定方法 水稻资源 |
| 收稿时间： | 2019-06-05 |
Study on the Method of Determining Digestible Protein Content and Screening of Rice Resources |
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| Chen Tingmu,Sun Zhiguang,Xing Yungao,Fang Zhaowei,Wang Baoxiang,Liu Yan,Xu Dayong.Study on the Method of Determining Digestible Protein Content and Screening of Rice Resources[J].Crops,2020,36(1):61-23. |
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| Authors: | Chen Tingmu Sun Zhiguang Xing Yungao Fang Zhaowei Wang Baoxiang Liu Yan Xu Dayong |
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| Institution: | Lianyungang Academy of Agricultural Sciences/Jiangsu Collaborative Innovation Center for Modern Crop Production, Lianyungang 222006, Jiangsu, China |
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| Abstract: | Rice is the main food crop and an important source of protein. The proteins of rice seed include non-digestible gliadin and digestible glutenin, albumin and globulin. A feasible method for determination of digestible protein content in rice was studied in 804 genotypes imported from International Rice Research Institute. Satisfactory results were obtained by adding 0.4% NaOH in 60% ethanol as the extraction solvent and using 300W ultrasound to extract digestible protein three times, simultaneously the average digestible protein of resources was measured. The average digestible protein content of 804 materials was 7.80%. It provided an effective technical means for the determination of digestible protein content in rice and screened a number of rice breeding materials with high and low digestible protein content. |
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| Keywords: | Rice protein Digestible protein Comassie brilliant blue G250 Determination method Rice resource |
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