| 低温等离子体诱变华山松种子效果研究 |
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| 引用本文： | 辛培尧,周军,唐军荣,张亚威,费希同,段安安.低温等离子体诱变华山松种子效果研究[J].广西农业科学,2014(4):619-622. |
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| 作者姓名： | 辛培尧 周军 唐军荣 张亚威 费希同 段安安 |
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| 作者单位： | [1]西南林业大学／国家林业局西南地区生物多样性保育重点实验室,昆明650224 [2]西南林业大学林学院,昆明650224 [3]中国环境科学研究院,北京100012 |
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| 基金项目： | 云南省应用基础研究基金项目（2009CD071）;国家林业局西南地区生物多样性保育重点实验室开放基金项目（BC2010F06） |
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| 摘 要： | 目的]探讨低温等离子体对华山松种子的诱变效果,为利用等离子体诱变进行植物遗传改良提供理论依据和实践指导.方法]用150、180、210、240、270及300W6种功率等离子体对华山松种子进行诱变处理,以不进行诱变处理作对照,测定诱变后种子的发芽率和发芽势;幼苗生长180 d后,观察幼苗苗高、地径和幼苗生长变异系数,分析等离子体对华山松幼苗生长的影响.结果]180、210、240W低温等离子体处理的华山松种子发芽率分别达70.67％、74.67％和72.00％,与对照（57.33％）的差异显著（P＜0.05,下同）;幼苗苗高分别为8.90、8.32和8.20 cm,与对照（7.83 cm）的差异显著;幼苗地径以210W处理最粗（1.88 mm）,与对照（1.62 mm）、300W处理（1.50 mm）差异显著.270、300 W低温等离子体处理的华山松种子发芽率分别为47.33％和28.67％,分别比对照低10.00和28.66个的百分点;幼苗苗高分别为7.11和7.14 cm,明显低于对照;300W处理的幼苗地径粗低于对照,且差异显著.300 W处理幼苗苗高和地径粗的诱变系数最高,分别为28.43％和24.67％.结论]270 W以下等离子体能促进华山松种子发芽和幼苗生长;利用300 W低温等离子体诱使华山松幼苗苗高和径粗发生变异的系数较高,便于进行华山松的良种选育.
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| 关 键 词： | 华山松 种子 低温等离子体 诱变效应 |
Preliminary trial report on mutation effects of low-temperature plasma on Pinus armandii Franch. |
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| XIN Pei-yao,ZHOU Jun,TANG Jun-rong,ZHANG Ya-wei,FEI Xi-tong,DUAN An-an.Preliminary trial report on mutation effects of low-temperature plasma on Pinus armandii Franch.[J].Guangxi Agricultural Sciences,2014(4):619-622. |
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| Authors: | XIN Pei-yao ZHOU Jun TANG Jun-rong ZHANG Ya-wei FEI Xi-tong DUAN An-an |
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| Institution: | 1Key Laboratory of Biodiversity Conservation in Southwest China/State Forestry Administration, Southwest Forestry University, Kunming 650224, China; 2College of Forestry, Southwest Forestry University, Kunming 650224, China; 3Chinese Research Academy of Environmental Sciences, Beijing 100012, China) |
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| Abstract: | Objective]Mutation effects of low-temperature plasma on P. armandii were studied to provide theoretical ref- erences and practical guide for improving plant heredity by plasma mutation. Method]P. armandii seeds were treated and in- duced by 6 different levels of plasma （150, 180, 210, 240, 270 and 300 W）, and then the germination rate of seeds and growth status of the seedling were measured （control： no mutation）. When seedlings grew for 180 d, their height, ground diam- eter and growth coefficient of variation were determined to evaluate the effects of plasma on P. armandii growth. ]Result]Seed germination rate in 180, 210 and 240 W treatment was 70.67%, 74.67% and 72.00%,respectively. Seed germination rate of the control was 57.33%, which was significantly different （P〈0.05）. Seedling height was 8.90, 8.32 and 8.20 era, which was significantly different from the control （7.83 cm）. The thickest ground diameter of 1.88 mm （in 210 W treatment） was signifi- cantly different from the control （1.62 mm）and the ground diameter in 300 W treatment （1.50 ram）. Seed germination rate in 270 and 300 W treatment was 47.33% and 28.67% down by 10.00% and 28.66%. Seedling height was 7.11 and 7.14 em, which was remarkably lower than the control. Seedling ground diameter in 300 W treatment was lower than the control and significantly different. Growth coefficient of variation of seedling height and ground diameter in 300 W treatment was the highest （28.43 and 24.67）. ]Conclusion]Plasma under 270 W promoted seed germination and seedling growth, while plas- ma above 300 W inhibited germination and seedling growth of P. armandii. P. armandii seedling height and growth coefficient of variation could be increased by low-temperature plasma （300 W）, and P. arraandii breeding is better in this way. |
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| Keywords: | Pinus armandii Franch seed low-temperature plasma mutation effect |
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