| 紫外-可见吸收光谱法测定芒果叶中的芒果苷和叶绿素 |
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| 引用本文： | 谢宇奇,林翠梧,赖清华,黄贵友.紫外-可见吸收光谱法测定芒果叶中的芒果苷和叶绿素[J].广西农业科学,2014(3):463-468. |
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| 作者姓名： | 谢宇奇 林翠梧 赖清华 黄贵友 |
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| 作者单位： | [1]广西大学化学化工学院,南宁530004 [2]百色学院化学与生命科学系,广西百色533000 |
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| 基金项目： | 广西自然科学基金项目（2010GXNSFA013038） |
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| 摘 要： | 【目的】建立可定量分析芒果叶中芒果苷和叶绿素含量的紫外-可见吸收光谱法，为今后综合开发利用广西芒果叶资源提供参考依据。【方法】采用紫外-可见吸收光谱法测定芒果叶中芒果苷和叶绿素的吸光值，然后通过芒果苷的线性回归方程和Arnon公式换算出芒果苷和叶绿素含量，同时测定广西百色市11个芒果品种叶片中的芒果苷和叶绿素含量。【结果】258.5 nm是芒果苷标准品和芒果叶提取物中芒果苷含量的最佳测定波长。采用紫外-可见吸收光谱法测定芒果苷含量时，其回归方程（y=0.0697x-0.0009）在4.120-41.200μg/mL范围内的线性关系良好（R2=0.9998），且准确性高、重复性好、检测结果稳定。以其测定广西百色市11个芒果品种叶片中的芒果苷和叶绿素，发现芒果苷含量范围为11.493-16.675μg/mL，其中以红象牙的含量较低，台牙和农院五号的含量较高；叶绿素总含量范围为4.83-8.77 mg/mL，其中以红贵妃和金煌芒的含量相对较高，四季蜜芒和农院五号的含量较低。【结论】紫外-可见吸收光谱法可用于评价芒果叶药材质量。
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| 关 键 词： | 芒果叶 叶绿素 芒果苷 紫外-可见吸收光谱法 含量测定 |
Determination of chlorophyll and mangiferin content in mango leaves by using UV-VIS Spectrum |
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| XIE Yu-qi,LIN Cui-wu,LAI Qing-hua,HUANG Gui-you.Determination of chlorophyll and mangiferin content in mango leaves by using UV-VIS Spectrum[J].Guangxi Agricultural Sciences,2014(3):463-468. |
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| Authors: | XIE Yu-qi LIN Cui-wu LAI Qing-hua HUANG Gui-you |
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| Institution: | 1School of Chemistry and Chemical Engineering ,Guangxi University, Nanning 530004, China; 2Department of Chemistry and Life Sciences, Baise University, Baise, Guangxi 533000, China) |
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| Abstract: | Objective]UV-VIS Spectrum was used to quantitatively analyze the content of mangiferin and chlorophyll in mango leaves in orde to provide references for comprehensive exploitation and utilization of Guangxi mango leaves resources in future. Method]Light absorption values of mangiferin and chlorophyll in mango leaves were measured using UV-VIS Spectrum, and then the contents of mangiferin and chlorophyll were conversed based on linear regression equation and Arnon formula. Meanwhile, the contents of mangiferin and chlorophyll in 11 kinds of mango leaves of Baise in Guangxi were studied.Result]The best wavelength for measuring mangiferin standard substance and the content of mangiferin in extract of mango leaves was 258.5 nm. When adopting UV-VIS Spectrum to determinate mangiferin content, the linear relationship （R2=0.9998） of the regression equation （y=0.0697x-0.0009） was good within the scope of 4.12-41.20μg/mL. The method was accurate with good repeatability and the test results were stable. The contents of mangiferin and chlorophyll in 11 kinds of mango leaves of Baise in Guangxi ranged from 11.493 to 16.675μg/mL. The content of Red ivory was low, while that of Taiya and Nongyuan No. 5 was higher. The total chlorophyll content ranged from 4.83 to 8.77 mg/mL. The contents of Guifeihong and Chiinhuang were comparatively high, while those of the Sijimi and Nongyuan No.5 were low. Conclusion]UV-VIS Spectrum could be used to evaluate the medicinal material quality of mango leaves. |
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| Keywords: | mango leaf chlorophyll mangiferin UV-VIS Spectrum content determination |
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