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| 两株水牛伊氏锥虫内转录间隔区基因序列的测定与系统进化关系 | |
| 引用本文： | 石云良,李健,黄维义,张为宇,张居作,付强,张晓溪,黄文忠,何国声.两株水牛伊氏锥虫内转录间隔区基因序列的测定与系统进化关系[J].中国预防兽医学报,2009,31(11). |
| 作者姓名： | 石云良 李健 黄维义 张为宇 张居作 付强 张晓溪 黄文忠 何国声 |
| 作者单位： | 1. 广西大学,动物科学技术学院,广西,南宁,530005 2. 广西百色农业学校,广西,百色,533000 3. 中国农业科学院上海兽医研究所,农业部动物寄生虫学重点实验室,上海,200232 |
| 摘 要： | 为研究从水牛分离到的伊氏锥虫广西株和湖北株的分类学地位,对2株伊氏锥虫的核糖体基因内转录间隔区(ITS1-5.8S-ITS2)基因进行了克隆和测序分析.用CLUSTALXl.83软件多重比对分析了序列差异,应用MEGA4.0软件绘制系统进化树.结果表明,这2株水牛伊氏锥虫的ITS1-5.8S-ITS2 rRNA基因扩增产物分别为1 102 bp和1 095 bp,序列存在多态性,其中广西株的ITS-1序列长为340 bp,ITS-2序列长为582 bp,湖北株的ITS-1序列长为339bp,ITS-2序列长为587 bp.系统进化分析显示,这2株伊氏锥虫分布在同一大枝的不同亚群中. |
| 关 键 词： | 水牛 伊氏锥虫 内转录间隔区(ITS) 序列分析 系统进化分析 |
Sequencing and phylogenetic analysis of internal transcribed spacer region(ITS)genes of two Trypanosoma evansi strains in Buffalo |
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| SHI Yun-liang,LI Jian,HUANG Wei-yi,ZHANG Wei-yu,ZHANG Ju-zuo,FU Qiang,ZHANG Xiao-xi,HUANG Wen-zhong,HE Guo-sheng.Sequencing and phylogenetic analysis of internal transcribed spacer region(ITS)genes of two Trypanosoma evansi strains in Buffalo[J].Chinese Journal of Preventive Veterinary Medicine,2009,31(11). | |
| Authors: | SHI Yun-liang LI Jian HUANG Wei-yi ZHANG Wei-yu ZHANG Ju-zuo FU Qiang ZHANG Xiao-xi HUANG Wen-zhong HE Guo-sheng |
| Abstract: | To study the taxonomy position of two Trypanosoma evansi strains from buffalos in Guangxi and Hubei provinces, the ITS (ITSI-5.8S-ITS2) gene of the T. Evansi were amplified by PCR and sequenced. Sequence analysis showed that the length of the ITS1-5.8S-ITS2 gene of the two T. Evansi strains varied between 1 102 bp and 1 095 bp. The length of ITSI and ITS2 of T. Evansi strain Guangxi was 340 bp and 582 bp respectively, in comparison to those of T. Evansi Hubei strain which was 339 bp and 587 bp respectively. The phylogenetic analysis indicated that these two T. Evansi strains were in the same branch but different subgroups based on ITS gene sequence. |
| Keywords: | buffalos T evansi ITS sequence analysis phylogenetic analysis |
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