| 鸡马立克氏病胶乳凝集和凝集抑制试验的研究 |
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| 引用本文： | 张峰,许兰菊.鸡马立克氏病胶乳凝集和凝集抑制试验的研究[J].河南农业大学学报,1993,27(2):170-175. |
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| 作者姓名： | 张峰 许兰菊 |
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| 作者单位： | 河南农业大学牧医系，河南农业大学牧医系，河南农业大学牧医系 郑州 450002，郑州 450002，郑州 450002 |
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| 摘 要： |
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| 关 键 词： | 马立克氏病 鸡病 |
Study on the Application of carboxylated Polystryrene Latex Microspheres to the Diagnosis and Immunodetection of Mark's Disease |
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| Zhang Feng Xu Lanju Yin Fengge.Study on the Application of carboxylated Polystryrene Latex Microspheres to the Diagnosis and Immunodetection of Mark''''s Disease[J].Journal of Henan Agricultural University,1993,27(2):170-175. |
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| Authors: | Zhang Feng Xu Lanju Yin Fengge |
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| Abstract: | Marek's Disease (MD) antigen collected from the feather pulp of infected chichens and antiserum to MD obtained from immunised rabbits were partially purifed by treatment with saturated ammonium sulfate solution, and were covalently bound on carboxylated ploystryrene latex microspheres respectively in the presence of carbodimide in order to preparate MD latex antibody which was used in latex antibody agglutination test (LAbAT) and its inhibition test(IAbAIT) to detect MD specific Ag of the pulp of feathers and MD latex Ag used in latex antigen agglutination test (LAgAT) and its inhibition test (IAgAIT) to detect Ab against MDV. The findings indicated that LAT and LAIT was of good repeatability and stability, high specificity and sensitivty. Compared with agar gel precipition test (AGPT), the sensitivity of MD LAgAT and MD LAbAT was 70.5 and 128 times that of AGPT respectively. Correlation coefficient between the titre of AGPT and that of MDLAgAT was 0.98(>r_(0.01). The titre of MDAg detecting with MDLAbAT was proportional to that of AGPT. Furthermore, MDLAg may be used for detecting maternal antibodies and the antibodies against Herpesvirus of Turkeys. It offered a special sensitive and rapid method for detecting the level and dynamic variation of MDAb. |
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| Keywords: | chicken Marek's Disease latex agglutination test latex agglutination inhibition test |
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