Seedborne Fusarium on Douglas-fir: Pathogenicity and seed stratification method to decrease Fusarium contamination |
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| Authors: | Paige E Axelrood Melody Neumann David Trotter Reed Radley Gwen Shrimpton John Dennis |
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| Institution: | (1) Forest Biotechnology Centre, B.C. Research Inc., 3650 Wesbrook Mall, V6S 2L2 Vancouver, B.C., Canada;(2) Nursery Extension Services, B.C. Ministry of Forests, 14275-96 Ave., V3V 7Z2 Surrey, B.C., Canada;(3) B.C. Hydro, E16-6911 Southpoint Drive, V3N 4X8 Burnaby, B.C., Canada;(4) Pacific Forestry Centre, Forestry Canada, 506 W. Burnside Rd., V8Z 1M5 Victoria, B.C., Canada |
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| Abstract: | Twelve Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlots from coastal British Columbia were assayed for seedborne Fusarium. All of the seedlots were contaminated with Fusarium. Percent of nonstratified seeds from individual seedlots harboring Fusarium ranged from 0.3% to 95.4%. Sixty-seven percent of the seedlots had Fusarium on less than 2% of the seeds. Post-stratification seedborne Fusarium levels were significantly less for running water imbibition compared to standing water imbibition. However, seedling growth at a container nursery was not consistently different for stratified seed imbibed initially in standing or running water. Fusarium disease symptoms were not observed in the nursery environment. The species of Fusarium isolated from seed were F. acuminatum, F. avenaceum, F. lateritium, F. moniliforme, F. oxysporum, F. poae and F. sambucinum. Twelve Fusarium isolates, comprising six species, were assessed for pathogenicity. Disease symptoms were observed after four weeks incubation and Fusarium isolates ranged in virulence from low to high. Fusarium oxysporum isolates were the most pathogenic. |
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| Keywords: | Fusarium disease Fusarium oxysporum seed sanitation Pseudotsuga menziesii seed imbibition |
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