| 从小麦EST序列中开发新的SSR引物 |
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| 引用本文： | 陈军方,任正隆,高丽锋,贾继增.从小麦EST序列中开发新的SSR引物[J].作物学报,2005,31(2):154-158. |
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| 作者姓名： | 陈军方 任正隆 高丽锋 贾继增 |
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| 作者单位： | 中国农业科学院作物品种资源研究所，农业部作物种质资源与生物技术重点开放实验室，北京10008 |
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| 基金项目： | 国家 8 63项目“小麦新型分子标记开发及抗病、优质、高产新品种培育”(2 0 0 1AA2 110 3 1) |
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| 摘 要： | 小麦EST数量的迅速增加为开发新的SSR标记提供了宝贵的数据资源，本实验从国际小麦族EST协作网（ITEC）上公布的10 380条EST序列中检索到444条含有SSR的序列，检出率为4.1%。其中含二核苷酸重复单元和三核苷酸重复单元的SSR-ESTs分别为34条（7.7%）和347条（78.0%）。利用这些SSR-ESTs序列共设计135对cSSR引物，其中82对在小
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| 关 键 词： | EST SSR-ESTs cSSR引物 小麦 |
| 收稿时间： | 2003-11-11 |
| 修稿时间： | 2003年11月11 |
Developing New SSR Markers from EST of Wheat |
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| CHEN Jun-fang,REN Zheng-long,GAO Li-feng,JIA Ji-Zeng.Developing New SSR Markers from EST of Wheat[J].Acta Agronomica Sinica,2005,31(2):154-158. |
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| Authors: | CHEN Jun-fang REN Zheng-long GAO Li-feng JIA Ji-Zeng |
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| Institution: | Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agriculture Sciences, Beijing 100081 |
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| Abstract: | The growing availability of EST sequences from wheat provides a potential valuable source of new SSR markers. In this study, 444 SSR-ESTs from 10 380 ESTs in the International Triticeae EST Cooperative (ITEC) database, representing 4.1% of the total number of ESTs were identified. Among them, 34 dinucleotide repeats and 347 trinucleotide repeats were found, accounting for 7.7% and 78.0% of the total SSR-ESTs respectively. 135 cSSR primers were designed to sequence flanking SSRs from 175 selected SSR-ESTs, of which 82 primers were efficient in wheat. 32 of the 82 cSSR primers were located on 18 wheat chromosomes except for 2D, 4B and 4D of 21 wheat chromosomes. |
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| Keywords: | EST SSR-ESTs cSSR primers Wheat |
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