| Study on the character of chitinase produced by Trichoderna spp. with measuring reducing sugar |
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| 作者姓名： | LIU Kai-qi XIANG Mei-mei LIU Ren ZENG Yong-san LI Hua JIANG Xin-yin ZHANG Yue-li |
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| 作者单位： | LIU Kai-qi（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）;XIANG Mei-mei（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）;LIU Ren（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）;ZENG Yong-san（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）;LI Hua（College of Plant Protection, Shandong Agric. Univ., Tai' an 271018, China）;JIANG Xin-yin（College of Plant Protection, Shandong Agric. Univ., Tai' an 271018, China）;ZHANG Yue-li（College of Plant Protection, Shandong Agric. Univ., Tai' an 271018, China） |
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| 摘 要： | A trusty and intuitionistic method for screening chitinase produced by Trichoderma spp. was developed. 38 isolates of Trichoderma spp. were cultured in liquid medium with chitin or colloidal chitin as the sole carbon source for 4 days. The supernatant of the fermented broth was mixed with colloidal chitin and heated in water-bath at 37℃ for 30 min, then 3,5-dinitrosalicylic acid reagent (DNS) was added to the mixture, and let them react for 10 min in water-bath. According to the different colour of the mixture, the isolates of Trichoderma spp. which can produce chitinase could be screened.……
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| 文章编号： | 1008-9209(2004)04-0430-01 |
Study on the character of chitinase produced by Trichoderna spp.with measuring reducing sugar |
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| LIU Kai-qi,XIANG Mei-mei,LIU Ren,ZENG Yong-san,LI Hua,JIANG Xin-yin,ZHANG Yue-li.Study on the character of chitinase produced by Trichoderna spp. with measuring reducing sugar[J].Journal of Zhejiang University(Agriculture & Life Sciences),2004,30(4):430-430. |
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| Authors: | LIU Kai-qi XIANG Mei-mei LIU Ren ZENG Yong-san LI Hua JIANG Xin-yin ZHANG Yue-li |
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| Abstract: | A trusty and intuitionistic method for screening chitinase produced by Trichoderma spp. was developed. 38 isolates of Trichoderma spp. were cultured in liquid medium with chitin or colloidal chitin as the sole carbon source for 4 days. The supernatant of the fermented broth was mixed with colloidal chitin and heated in water-bath at 37℃ for 30 min, then 3,5-dinitrosalicylic acid reagent (DNS) was added to the mixture, and let them react for 10 min in water-bath. According to the different colour of the mixture, the isolates of Trichoderma spp. which can produce chitinase could be screened. |
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| Keywords: | Trichoderma chitinase reducing sugar |
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