| 鸡传染性法氏囊病病毒VP2蛋白GFP融合分子在COS7细胞内的表达 |
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| 引用本文： | 李姣,张红,张改平,王爱萍,易明林,郅玉宝,鲁琨,张丽萍.鸡传染性法氏囊病病毒VP2蛋白GFP融合分子在COS7细胞内的表达[J].中国畜牧兽医,2008,35(7):40-42. |
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| 作者姓名： | 李姣 张红 张改平 王爱萍 易明林 郅玉宝 鲁琨 张丽萍 |
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| 作者单位： | 1. 河南省动物免疫学重点实验室，郑州 450002； 2. 郑州大学，郑州 450001；3. 河南教育学院，郑州 450003 |
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| 摘 要： | 将鸡传染性法氏囊病病毒（IBDV）VP2蛋白基因插入增强型绿色荧光表达载体pEGFP-C1中，构建了真核表达载体pEGFP-VP2。PCR与酶切鉴定结果表明VP2基因成功插入到表达载体pEGFP-C1中。脂质体法转染COS7细胞后，荧光显微镜检测到了GFP-VP2融合蛋白的表达。用200 μg/ml的G418成功筛选到了稳定表达GFP-VP2融合蛋白的细胞系，表达蛋白经镍亲和柱得到了纯化。
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| 关 键 词： | 鸡 传染性法氏囊病病毒 VP2蛋白 真核表达 GFP融合蛋白 |
Expression of VP2 Protein of Infectious Bursal Disease Virus Fused with GFP in COS7 Cells |
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| LI Jiao,ZHANG Hong,ZHANG Gai-ping,WANG Ai-ping,YI Ming-lin,ZHI Yu-bao,LU Kun,ZHANG Li-ping.Expression of VP2 Protein of Infectious Bursal Disease Virus Fused with GFP in COS7 Cells[J].China Animal Husbandry & Veterinary Medicine,2008,35(7):40-42. |
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| Authors: | LI Jiao ZHANG Hong ZHANG Gai-ping WANG Ai-ping YI Ming-lin ZHI Yu-bao LU Kun ZHANG Li-ping |
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| Institution: | Henan Key Laboratory for Animal Immunology, Zhengzhou 450002, China; 2.Zhengzhou University, Zhengzhou 450001, China; 3.Henan Educational Institute, Zhengzhou 450003, China |
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| Abstract: | VP2 gene of infectious bursal disease virus(IBDV) was inserted into enhanced GFP expression vector pEGFP-C1. The recombinant eukaryotic expression vector pEGFP-VP2 was constructed, and detected by endonuclease digestion and PCR , and then was ransfected to COS7 cells by lipid reagent. After infecting, green fluorescence was observed under fluorescent microscope. The results indicates that GFP-VP2 fusion protein was successfully expressed in COS7 cells. Stable expressing cells was selected by 200 μg/ml G418, and GFP-VP2 fusion protain was purified by Ni His affinity column. |
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| Keywords: | chicken IBDV VP2 protein eukaryotic expression GFP fusion protein |
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